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Titolo:
STUDIES ON THE PLATELET SURFACE-ASSOCIATED VON-WILLEBRAND-FACTOR
Autore:
MARTIN SE; JOHNSON MM; ABEL RF;
Indirizzi:
MED CTR DELAWARE,BLOOD BANK,DEPT PATHOL,4755 OGLETOWN STANTON RD,POB 6001 NEWARK DE 19718 MED CTR DELAWARE,TRANSFUS SERV NEWARK DE 00000 MED CTR DELAWARE,DEPT MED NEWARK DE 00000
Titolo Testata:
American journal of hematology
fascicolo: 1, volume: 46, anno: 1994,
pagine: 1 - 8
SICI:
0361-8609(1994)46:1<1:SOTPSV>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
VIII-VONWILLEBRAND-FACTOR; THROMBASTHENIC PLATELETS; STIMULATED PLATELETS; GLYCOPROTEIN-IIB; FACTOR-VA; DISEASE; LOCALIZATION; THROMBIN; PROTEIN; BINDING;
Keywords:
PLATELETS; VON WILLEBRAND FACTOR; ALPHA-GRANULES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
S.E. Martin et al., "STUDIES ON THE PLATELET SURFACE-ASSOCIATED VON-WILLEBRAND-FACTOR", American journal of hematology, 46(1), 1994, pp. 1-8

Abstract

The contribution of platelets to the prevention and control of bleeding depends not only on the plasma and vessel wall von Willebrand (vW) factor, but also on the vW factor originating from platelets. Althoughplatelet vW factor is mainly stored in the alpha-granules, small amounts of vW factor have been detected on the platelet surface under conditions without direct stimulation. This communication focuses on the small amount of vW factor associated with the surface of platelets. EDTA-washed platelets in a calcium-free medium containing prostaglandin E, (PGE,) were exposed to anti-vW factor F(ab')(2) fragments. The platelet-associated anti-vW factor antibodies were separated from the free fragments by gel chromatography. Normal platelets were compared to platelets from patients with Bernard Soulier syndrome, Glanzmann's thrombasthenia, and Hermansky-Pudlak syndrome. These experiments showed that, in the absence of extracellular calcium, vW factor can be detected on the surface of washed platelets independent of glycoproteins Ib and IIb-IIIa, and despite markedly reduced intracellular calcium content. This particular platelet surface expression of vW factor is probably the result of disturbing the platelet during the in vitro manipulation.von Willebrand factor is an extremely important element in the multiple molecular interactions required to stop and anchor the platelet in areas of high flow rates. Mechanical perturbation of platelets leadingto surface expression of the vW factor helps increase its availability for such critical interactions. (C) 1994 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 11:45:50