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Titolo:
REARRANGEMENT OF MESSENGER-RNAS FOR COSTAMERE PROTEINS DURING COSTAMERE DEVELOPMENT IN CULTURED SKELETAL-MUSCLE FROM CHICKEN
Autore:
MORRIS EJ; FULTON AB;
Indirizzi:
UNIV IOWA,DEPT BIOCHEM IOWA CITY IA 52242 UNIV IOWA,DEPT BIOCHEM IOWA CITY IA 52242
Titolo Testata:
Journal of Cell Science
, volume: 107, anno: 1994,
parte:, 3
pagine: 377 - 386
SICI:
0021-9533(1994)107:<377:ROMFCP>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
MYOTUBES INVIVO; VIMENTIN; DESMIN; VINCULIN; SITES; CELLS; TITIN; LOCALIZATION; EXPRESSION; MYOGENESIS;
Keywords:
IN-SITU HYBRIDIZATION; COSTAMERE; MYOFIBRILLOGENESIS; INTERMEDIATE FILAMENTS; MESSENGER RNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
E.J. Morris e A.B. Fulton, "REARRANGEMENT OF MESSENGER-RNAS FOR COSTAMERE PROTEINS DURING COSTAMERE DEVELOPMENT IN CULTURED SKELETAL-MUSCLE FROM CHICKEN", Journal of Cell Science, 107, 1994, pp. 377-386

Abstract

Mature skeletal myofibrils are surrounded by costameres, ribs of metavinculin, vinculin, intermediate filaments, and other proteins that connect the myofibril to the extracellular matrix. Costameres have recently been shown to be the sites at which the forces generated by the myofibril are transduced laterally into the extracellular matrix. We observed costameres developing in cultured skeletal muscles, grown in micromass culture from cells taken from embryonic chicken leg. We detected proteins by immunofluorescence and mRNA by in situ hybridization. Antibody and probe signals were imaged by laser scanning confocal microscopy. Antibody to vimentin protein is first detected in stripes in register with the Z line of the myofibril, at -day 12 after fusion; soon thereafter probe to vimentin mRNA is also detected in the same stripes. Optical sections indicate that vimentin mRNA and protein are very close, no more than 0.1 mm apart and possibly in immediate contact. Antibody to vimentin is detected in stripes only in cells that twitch spontaneously. Antibodies and probes to desmin and vinculin protein and mRNA are next detected in stripes of the same periodicity, at approximately day 17 after fusion. Vinculin protein (but not mRNA) is detected at focal contacts much earlier in development. Controls for bleed through of fluorescence, RNase H sensitivity, hybridization without probe, and binding to the myofibril all gave appropriate results. Probes to glyceraldehyde-3-phosphate dehydrogenase, a glycolytic enzyme, stained diffusely and did not associate with the myofibril. These results showthat components of the costamere arrive at the structure in a definedsequence, and that mRNA organization is a conspicuous, precise and temporally controlled aspect of costamere development. These results mayhave wider implications. In these cells, some mRNAs are positioned with submicrometer precision in space and differentially over time. Particular mRNAs differ in the time and place of such positioning. This implies both that cellular structures provide physical cues for such positioning and that mRNA contains information that interacts with such cues in a message-specific manner. If such precision in mRNA location is found in other somatic cells, it could have significant implicationsfor the ways in which cells generate and maintain cellular structures.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 06:21:48