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Titolo:
INSERTION OF A NOVEL DNA-SEQUENCE, IS-1186, UPSTREAM OF THE SILENT CARBAPENEMASE GENE CFIA, PROMOTES EXPRESSION OF CARBAPENEM RESISTANCE INCLINICAL ISOLATES OF BACTEROIDES-FRAGILIS
Autore:
PODGLAJEN I; BREUIL J; COLLATZ E;
Indirizzi:
UNIV PARIS 06,MICROBIOL MED LAB,5 RUE ECOLE MED F-75270 PARIS 06 FRANCE UNIV PARIS 06,MICROBIOL MED LAB,5 RUE ECOLE MED F-75270 PARIS 06 FRANCE CTR HOSP INTERCOMMUNAL F-94190 VILLENEUVE ST GEO FRANCE
Titolo Testata:
Molecular microbiology
fascicolo: 1, volume: 12, anno: 1994,
pagine: 105 - 114
SICI:
0950-382X(1994)12:1<105:IOANDI>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
COMPLETE NUCLEOTIDE-SEQUENCE; METALLO-BETA-LACTAMASE; ESCHERICHIA-COLI; IMIPENEM RESISTANCE; MOLECULAR ANALYSIS; ELEMENT IS1; STRAINS; IDENTIFICATION; CLONING; CEFOXITIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
I. Podglajen et al., "INSERTION OF A NOVEL DNA-SEQUENCE, IS-1186, UPSTREAM OF THE SILENT CARBAPENEMASE GENE CFIA, PROMOTES EXPRESSION OF CARBAPENEM RESISTANCE INCLINICAL ISOLATES OF BACTEROIDES-FRAGILIS", Molecular microbiology, 12(1), 1994, pp. 105-114

Abstract

A small number of isolates of Bacteroides fragilis, an anaerobic pathogen of the human intestinal flora, carries a copy (or copies) of the carbapenem-resistance gene, cfiA, which may be silent or expressed. Wehave studied the mechanism of activation of the frequently silent gene in in vitro-selected mutants and in clinical isolates. In both typesof strains, activation was observed as the consequence of the insertion, at several possible sites, of a novel 1.3 kb insertion sequence, IS1186, immediately upstream of the carbapenemase gene. IS1186 has two open reading frames, on opposite strands, with coding capacities for a41.2 kDa (ORF1) and a 22.5 kDa (ORF2) protein. The 41.2 kDa protein has homology with some proteins predicted from open reading frames of IS elements or DNA direct repeats of aerobic, but not anaerobic, Gram-negative bacteria. Upon insertion, transcription of cfiA was found to be driven from a promoter identified on the right end of IS1186. In oneinstance, insertion occurred into the putative ribosome-binding site of cfiA, leaving intact the tetranucleotide AGAA which is concluded tobe a fully functional ribosome-binding site. Between 3 and 14 copies of IS 1186 were detected per genome and the element was found, within the species B. fragilis, almost exclusively in the subgroup carrying the cfiA gene.

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Documento generato il 30/05/20 alle ore 15:24:46