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Titolo:
ANALYSIS OF THE ANTIGENIC PROFILE OF MEASLES-VIRUS HEMAGGLUTININ IN MICE AND HUMANS USING OVERLAPPING SYNTHETIC PEPTIDES
Autore:
OBEID OE; PARTIDOS CD; STEWARD MW;
Indirizzi:
UNIV LONDON LONDON SCH HYG & TROP MED,DEPT CLIN SCI,MOLEC IMMUNOL UNIT,KEPPEL ST LONDON WC1E 7HT ENGLAND
Titolo Testata:
Virus research
fascicolo: 1, volume: 32, anno: 1994,
pagine: 69 - 84
SICI:
0168-1702(1994)32:1<69:AOTAPO>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
MONOCLONAL-ANTIBODIES; HEMAGGLUTININ; PROTEINS; PROTECTION; EPITOPES; IMMUNIZATION; DETERMINANTS; STRATEGIES; VACCINES; LOCATION;
Keywords:
MEASLES VIRUS; HEMAGGLUTININ; SYNTHETIC PEPTIDE; B-CELL EPITOPE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
O.E. Obeid et al., "ANALYSIS OF THE ANTIGENIC PROFILE OF MEASLES-VIRUS HEMAGGLUTININ IN MICE AND HUMANS USING OVERLAPPING SYNTHETIC PEPTIDES", Virus research, 32(1), 1994, pp. 69-84

Abstract

In this study, a panel of 55 synthetic peptides representing 92.2% ofthe haemagglutinin (H) glycoprotein of measles virus (MV) were used to study the antigenic profile of the H molecule of anti-MV antibodies raised in mice and late convalescent human sera. In addition the immunogenicity of these peptides was tested in two mouse strains. Mouse anti-MV antibodies had different fine specificity of binding to the peptides depending on the mouse strain. Thus in BALB/c (H-2(d)) mice, anti-MV antibodies recognised six peptides representing residues 103-117; 123-237; 242-255; 293-307 and 463-477. In TO (H-2(S)) mice, anti-MV antibodies recognised peptides representing residues 49-72 and 463-477. When the immunogenicity of the peptides was tested, 29 were immunogenicin BALB/c mice and 34 were immunogenic fn TO mice. Several of the anti-peptide antisera were found to cross-react with MV, depending on thesolid phase assay system used but none were able to inhibit virus infectivity in vitro. The reactivity of a panel of late convalescent human sera with the peptides was heterogeneous and the extent of the binding to the peptides was related to the titre of anti-MV. However, humansera recognized certain peptides more frequently than others, in particular peptides at the carboxyl-terminus.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 18:35:55