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Titolo:
INTRACELLULAR IMMUNIZATION OF MOSQUITO CELLS TO LACROSSE VIRUS USING A RECOMBINANT SINDBIS VIRUS VECTOR
Autore:
POWERS AM; OLSON KE; HIGGS S; CARLSON JO; BEATY BJ;
Indirizzi:
COLORADO STATE UNIV,DEPT MICROBIOL,ARTHROPOD BORNE ANIM DIS RES LAB FT COLLINS CO 80523 COLORADO STATE UNIV,DEPT MICROBIOL,ARTHROPOD BORNE ANIM DIS RES LAB FT COLLINS CO 80523
Titolo Testata:
Virus research
fascicolo: 1, volume: 32, anno: 1994,
pagine: 57 - 67
SICI:
0168-1702(1994)32:1<57:IIOMCT>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENE-EXPRESSION; ANTISENSE RNA; TRANSGENIC PLANTS; COAT PROTEIN; INTERFERENCE; INFECTION; PROTECTION; RESISTANCE; SUPERINFECTION; INHIBITION;
Keywords:
SINDBIS; LACROSSE; INTERFERENCE; ANTISENSE; RNA VIRUS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
A.M. Powers et al., "INTRACELLULAR IMMUNIZATION OF MOSQUITO CELLS TO LACROSSE VIRUS USING A RECOMBINANT SINDBIS VIRUS VECTOR", Virus research, 32(1), 1994, pp. 57-67

Abstract

A cDNA of the small RNA genome segment of La Crosse (LAC) virus was inserted, in an antisense orientation, into a double subgenomic Sindbis(dsSIN) virus expression vector generating pTE/3'2J/ANTI-S (15,000bp). In vitro transcription of the pTE/3'2J/ANTI-S template generated genomic RNA that was electrotransfected into BHK-21 cells to produce virus. Northern blot analysis of RNA isolated from infected Aedes albopictus (C6/36) cells showed that the TE/3'2J/ANTI-S virus produced a subgenomic mRNA of the appropriate size, indicating transcription of the LAC cDNA segment. C6/36 cells were infected with either TE/3'2J/ANTI-S, TE/3'2J (a dsSIN virus with no LAC insert), or wild type Sindbis (SIN,strain AR339) viruses and subsequently challenged with LAC virus. LACvirus titers were determined using a capture antibody ELISA. Mosquitocells infected with TE/3'2J/ ANTI-S virus yielded at least 4 log(10) TCID50/ml less LAC virus than cells infected with either TE/3'2J or AR339 SIN viruses. The use of the infectious SIN virus expression vectors provides a novel approach for high level cytoplasmic expression of genes or sequences of interest in arthropod cells, and for evaluating strategies for intracellular immunization against arboviruses.

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Documento generato il 01/12/20 alle ore 08:12:33