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Titolo:
ANALYSIS OF THE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 ENVELOPE PROTEIN-INTERACTION WITH THE CD4 HOST-CELL RECEPTOR
Autore:
MALVOISIN E; WILD F;
Indirizzi:
INST PASTEUR,AVE TONY GARNIER F-69365 LYON 07 FRANCE
Titolo Testata:
Journal of General Virology
, volume: 75, anno: 1994,
parte:, 4
pagine: 839 - 847
SICI:
0022-1317(1994)75:<839:AOTHTE>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECOMBINANT SOLUBLE CD4; OLIGOMERIC STRUCTURE; SYNCYTIUM FORMATION; GLYCOPROTEIN; INFECTION; HIV; BINDING; FUSION; DOMAIN; GP120;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
E. Malvoisin e F. Wild, "ANALYSIS OF THE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 ENVELOPE PROTEIN-INTERACTION WITH THE CD4 HOST-CELL RECEPTOR", Journal of General Virology, 75, 1994, pp. 839-847

Abstract

secreted form of human immunodeficiency virus type I (HIV-I) envelopeglycoprotein (gp160s), expressed in HeLa cells from a vaccinia virus recombinant was analysed by velocity-gradient centrifugation and chemical cross-linking. We showed that gp160s existed predominantly as a dimer, but higher forms corresponding to trimers and tetramers were alsofound. Soluble CD4 (sCD4) and native CD4 expressed by recombinant vaccinia viruses were analysed by sucrose-gradient sedimentation alone orafter complexing with gp160s. The sCD4 sedimented in sucrose gradients as a monomer, whereas after solubilization the native CD4 was in a dimeric state. Both forms of CD4 were able to form complexes when incubated with gp160s. In the case of the sCD4, the M(r) corresponded to a (sCD4)(2)-(gp160s)(2) complex, whereas with CD4 the complexes were of a greater order of magnitude. HIV gp120 was secreted into the medium in a monomeric state. With sCD4 it gave a one-to-one complex, whereas with the native CD4 high M(r) complexes were formed. The importance of the oligomeric state of the virus- and cell-receptor proteins are discussed regarding their avidities.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 00:39:35