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Titolo:
AFRICAN BUFFALO SERUM CONTAINS NOVEL TRYPANOCIDAL PROTEIN
Autore:
REDUTH D; GROOTENHUIS JG; OLUBAYO RO; MURANJAN M; OTIENOOMONDI FP; MORGAN GA; BRUN R; WILLIAMS DJL; BLACK SJ;
Indirizzi:
OHIO STATE UNIV,DEPT MICROBIOL,484 W 12TH AVE COLUMBUS OH 43210 OHIO STATE UNIV,DEPT MICROBIOL COLUMBUS OH 43210 SWISS TROP INST CH-4051 BASEL SWITZERLAND INT LAB RES ANIM DIS NAIROBI KENYA NATL VET RES CTR,KENYA AGR RES INST KABETE KENYA INT CTR INSECT PHYSIOL & ECOL NAIROBI KENYA
Titolo Testata:
The Journal of eukaryotic microbiology
fascicolo: 2, volume: 41, anno: 1994,
pagine: 95 - 103
SICI:
1066-5234(1994)41:2<95:ABSCNT>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
HIGH-DENSITY LIPOPROTEIN; TRYPANOSOMA-BRUCEI; MONOCLONAL-ANTIBODIES; CATTLE; MICE; SUSCEPTIBILITY; IDENTIFICATION; INFECTIONS; CONGOLENSE; ALLOTYPES;
Keywords:
CAPE BUFFALO; TRYPANOCIDAL SERUM PROTEIN; TRYPANOSOMIASIS; VSG-SPECIFIC ANTIBODY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
D. Reduth et al., "AFRICAN BUFFALO SERUM CONTAINS NOVEL TRYPANOCIDAL PROTEIN", The Journal of eukaryotic microbiology, 41(2), 1994, pp. 95-103

Abstract

The high ability of African buffalo, as compared to domestic cattle, to control infections with Trypanosoma brucei brucei ILTat 1.4 organisms did not correlate with the timing or magnitude of parasite surface coat-specific antibody responses and may have resulted from the constitutive presence in buffalo blood of a novel trypanocidal factor. Buffalo plasma and serum contained material that killed bloodstream stage T. b. brucei, T. b. rhodesiense, T. b. gambiense, T. evansi, T. congolense, and T. vivax organisms during four h of incubation at 37 degrees C in vitro. Serum from eland was also trypanocidal whereas serum from oryx, waterbuck, yellow-back duiker, cattle, horse, sheep, goat, mouse, rat, and rabbit was not trypanocidal. The buffalo serum trypanocidalmaterial was not lipoprotein, or Igc, and had the following properties: 1) a density of >1.24 g/ml determined by flotation ultracentrifugation; 2) insolubility in 50% saturated ammonium sulphate; 3) non-reactivity with anti-bovine IgM, and anti-bovine IgG; 4) non-reactivity withprotein G, and protein A; 5) a relative molecular mass of 152 kDa determined by chromatography on Sephacryl S 300, and of 133 kDa determined by chromatography of the 50% SAS cut of IgG-depleted buffalo serum on Superose 12; 6) no associated cholesterol; and 7) inactivation by digestion with proteinase K that was immobilized on agarose.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 17/01/21 alle ore 18:15:09