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Titolo:
ANTITETANUS TOXOID ANTIBODY-PRODUCTION AFTER MISMATCHED T-CELL-DEPLETED BONE-MARROW TRANSPLANTATION
Autore:
BENKERROU M; WARA DW; ELDER M; DROR Y; MERINO A; COLOMBE BW; GAROVOY M; COWAN MJ;
Indirizzi:
UNIV CALIF SAN FRANCISCO,DEPT PEDIAT,DIV BONE MARROW TRANSPLANT,505 PARNASSUS AVE SAN FRANCISCO CA 94143 UNIV CALIF SAN FRANCISCO,DEPT PEDIAT,DIV BONE MARROW TRANSPLANT,505 PARNASSUS AVE SAN FRANCISCO CA 94143 UNIV CALIF SAN FRANCISCO,DEPT SURG,DIV IMMUNOGENET & TRANSPLANTAT SANFRANCISCO CA 94143 UNIV CALIF SAN FRANCISCO,DEPT PEDIAT,DIV IMMUNOL SAN FRANCISCO CA 94143
Titolo Testata:
Journal of clinical immunology
fascicolo: 2, volume: 14, anno: 1994,
pagine: 98 - 106
SICI:
0271-9142(1994)14:2<98:ATAAMT>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEVERE COMBINED IMMUNODEFICIENCY; INVIVO BOOSTER IMMUNIZATION; HUMAN B-CELLS; LYMPHOCYTES-B; INVITRO; IL-6; HYBRIDIZATION; INDUCTION; BIOLOGY; DISEASE;
Keywords:
B-CELL; BONE MARROW TRANSPLANT; T-CELL DEPLETION; SEVERE COMBINED IMMUNODEFICIENCY DISEASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
M. Benkerrou et al., "ANTITETANUS TOXOID ANTIBODY-PRODUCTION AFTER MISMATCHED T-CELL-DEPLETED BONE-MARROW TRANSPLANTATION", Journal of clinical immunology, 14(2), 1994, pp. 98-106

Abstract

We explored B-cell function after tetanus toxoid (TT) immunization in12 children with severe combined immunodeficiency disease or leukemiawho were long-term survivors of an HLA-matched sibling or haplocompatible T cell-depleted parental bone marrow transplant (BMT), 10 of their healthy donors, and 13 normal controls. Specific in vivo and in vitro anti-TT antibody (Ab) production were measured by ELISA. We studied donors' and recipients' peripheral blood mononuclear cells (PBMC) and mixed E- (non-T cells) and E+ cells (T cells) spontaneously and after stimulation by TT in the absence or presence of interleukin-2 (IL-2), IL-4, and IL-6. Five of the 12 patients and all donors and controls responded with in vivo anti-TT Ab. In vitro anti-TT Ab production correlated with the in vivo response. All seven of the nonresponders were either fully engrafted or mixed chimeras (donor T cells but autologous Bcells and monocytes). We could not identify a T-cell defect in four of the five nonresponders who were tested. In contrast, E- cells from three of three responders cooperated with fresh donor E+ cells even when they shared only one HLA haplotype. In three of seven nonresponders,in vitro anti-TT Ab production was restored after the addition of IL-4 or IL-6 but not IL-2. Our results suggest that the humoral immunodeficiency that exists post mismatched T cell-depleted BMT is either a B-cell, a monocyte, or a B-cell/T-cell cooperation defect which, in somepatients, may be correctible with the addition of a cytokine. Also, it is not necessary to engraft donor B cells to achieve normal antibodyresponses and the ability to respond does not appear to correlate with pretransplant chemotherapy.

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Documento generato il 11/07/20 alle ore 10:28:31