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Titolo:
MODULATION OF SURFACTANT PROTEIN-D EXPRESSION BY GLUCOCORTICOIDS IN FETAL-RAT LUNG
Autore:
MARIENCHECK W; CROUCH E;
Indirizzi:
WASHINGTON UNIV,JEWISH HOSP ST LOUIS,MED CTR,DEPT PATHOL,216 S KINGSHIGHWAY ST LOUIS MO 63110 WASHINGTON UNIV,JEWISH HOSP ST LOUIS,MED CTR,DEPT PATHOL ST LOUIS MO 63110
Titolo Testata:
American journal of respiratory cell and molecular biology
fascicolo: 4, volume: 10, anno: 1994,
pagine: 419 - 429
SICI:
1044-1549(1994)10:4<419:MOSPEB>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
INVIVO DEXAMETHASONE TREATMENT; PULMONARY SURFACTANT; HORMONAL-CONTROL; GENE-EXPRESSION; FATTY-ACID; II CELLS; SP-A; SP-B; SP-C; RNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
W. Mariencheck e E. Crouch, "MODULATION OF SURFACTANT PROTEIN-D EXPRESSION BY GLUCOCORTICOIDS IN FETAL-RAT LUNG", American journal of respiratory cell and molecular biology, 10(4), 1994, pp. 419-429

Abstract

The production of pulmonary surfactant protein D (SP-D) increases abruptly during late gestation, and the accumulation of this protein in lung tissue is increased following the exposure of fetal rats to glucocorticoids in utero. To examine the regulation of these events, we administered dexamethasone (Dex; 1 mg/kg/day intramuscularly for 3 days), or saline, to timed-pregnant rats and harvested the lungs on days 19 to 21 of gestation. Samples of pooled fetal lungs were analyzed for SP-D protein, mRNA, and gene transcription by immunoblot, Northern hybridization, and nuclear run-off transcription assays. Lungs from 19 day controls showed barely detectable levels of SP-D gene transcription andnegligible accumulation of SP-D message. However, SP-D transcription and the accumulation of SP-D mRNA and protein were readily detected inlungs from 19 day Dex-treated rats. Dexamethasone also caused dose- and time-dependent increases in SP-D protein and mRNA accumulation in 19 day fetal lung explants. Immunohistochemistry of control 19 day lungusing antibodies to rat SP-D showed only weak labeling of a small number of airway epithelial cells. By contrast, Dex-exposed fats showed strong staining of columnar and cuboidal epithelial cells lining airways and epithelial tubules and cuboidal cells lining primitive air sacs. In situ hybridization assays showed similar alterations in the number, intensity, and distribution of labeled epithelial cells in 19 day Dex-exposed lungs and demonstrated labeling of alveolar type II and nonciliated columnar cells in adult lung. These data indicate that the accelerated lung maturation accompanying glucocorticoid exposure in uterois associated with a precocious increase in SP-D gene transcription and protein production by pulmonary epithelial cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 12:25:45