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Titolo:
A GROUP-III TWINTRON ENCODING A MATURASE-LIKE GENE EXCISES THROUGH LARIAT INTERMEDIATES
Autore:
COPERTINO DW; HALL ET; VANHOOK FW; JENKINS KP; HALLICK RB;
Indirizzi:
UNIV ARIZONA,DEPT BIOCHEM TUCSON AZ 85721 UNIV ARIZONA,DEPT BIOCHEM TUCSON AZ 85721 UNIV ARIZONA,DEPT MOLEC & CELLULAR BIOL TUCSON AZ 85721
Titolo Testata:
Nucleic acids research
fascicolo: 6, volume: 22, anno: 1994,
pagine: 1029 - 1036
SICI:
0305-1048(1994)22:6<1029:AGTEAM>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
GRACILIS CHLOROPLAST DNA; YEAST MITOCHONDRIA; SPLICING INVITRO; PHOTOSYSTEM-II; INTRON; EUGLENA; PROTEINS; EVOLUTION; SEQUENCES; SPLICEOSOME;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
48
Recensione:
Indirizzi per estratti:
Citazione:
D.W. Copertino et al., "A GROUP-III TWINTRON ENCODING A MATURASE-LIKE GENE EXCISES THROUGH LARIAT INTERMEDIATES", Nucleic acids research, 22(6), 1994, pp. 1029-1036

Abstract

The 1605 bp intron 4 of the Euglena gracilis chloroplast psbC gene was characterized as a group III twintron composed of an internal 1503 nt group III intron with an open reading frame of 1374 nt (ycf13, 458 amino acids), and an external group III intron of 102 nt. Twintron excision proceeds by a sequential splicing pathway. The splicing of the internal and external group III introns occurs via lariat intermediates. Branch sites were mapped by primer extension RNA sequencing. The unpaired adenosines in domains VI of the internal and external introns arecovalently linked to the 5' nucleotide of the intron via 2'-5' phosphodiester bonds. This bond is susceptible to hydrolysis by the debranching activity of the HeLa nuclear S100 fraction. The internal intron and presumptive ycfl3 mRNA accumulates primarily as a linear RNA, although a lariat precursor can also be detected. The ycfl3 gene encodes a maturase-like protein that may be involved in group III intron metabolism.

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Documento generato il 05/12/20 alle ore 01:12:32