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Titolo:
STRUCTURES OF A LEGUME LECTIN COMPLEXED WITH THE HUMAN LACTOTRANSFERRIN N2 FRAGMENT, AND WITH AN ISOLATED BIANTENNARY GLYCOPEPTIDE - ROLE OF THE FUCOSE MOIETY
Autore:
BOURNE Y; MAZURIER J; LEGRAND D; ROUGE P; MONTREUIL J; SPIK G; CAMBILLAU C;
Indirizzi:
FAC MED SECTEUR NORD MARSEILLE,CRISTALLOG & CRISTALLISAT MACROMOLEC BIOL LAB,CNRS,URA 1296 F-13916 MARSEILLE 20 FRANCE UNIV LILLE 1,CHIM BIOL LAB,CNRS,UMR 111 F-59655 VILLENEUVE DASCQ FRANCE FAC SCI PHARMACEUT TOULOUSE,CNRS,DEPT BIOL STRUCT & INGN PROT F-31062TOULOUSE FRANCE
Titolo Testata:
Structure
fascicolo: 3, volume: 2, anno: 1994,
pagine: 209 - 219
SICI:
0969-2126(1994)2:3<209:SOALLC>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
X-RAY STRUCTURE; BINDING SITE; 3-DIMENSIONAL STRUCTURES; ACETYLLACTOSAMINE TYPE; CARBOHYDRATE-BINDING; CONCANAVALIN-A; SPECIFICITY; RESOLUTION; GLYCANS; OLIGOSACCHARIDES;
Keywords:
FUCOSE; GLYCOSYLPROTEIN; LECTIN; OLIGOSACCHARIDE X-RAY STRUCTURE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
Y. Bourne et al., "STRUCTURES OF A LEGUME LECTIN COMPLEXED WITH THE HUMAN LACTOTRANSFERRIN N2 FRAGMENT, AND WITH AN ISOLATED BIANTENNARY GLYCOPEPTIDE - ROLE OF THE FUCOSE MOIETY", Structure, 2(3), 1994, pp. 209-219

Abstract

Background: Lectins mediate cell-cell interactions by specifically recognizing oligosaccharide chains. Legume lectins serve as mediators for the symbiotic interactions between plants and nitrogen-fixing microorganisms, an important process in the nitrogen cycle. Lectins from theViciae tribe have a high affinity for the fucosylated biantennary N-acetyllactosamine-type glycans which are to be found in the majority ofN-glycosylproteins. While the structures of several lectins complexedwith incomplete oligosaccharides have been solved, no previous structure has included the complete glycoprotein. Results: We have determined the crystal structures of Lathyrus ochrus isolectin II complexed with the N2 monoglycosylated fragment of human lactotransferrin (18 kDa) and an isolated glycopeptide (2.1 kDa) fragment of human lactotransferrin (at 3.3 angstrom and 2.8 angstrom resolution, respectively). Comparison between the two structures showed that the protein part of the glycoprotein has little influence on either the stabilization of the complex or the sugar conformation. In both cases the oligosaccharide adopts the same extended conformation. Besides the essential mannose moiety of the monosaccharide-binding site, the fucose-1' of the core has alarge surface of interaction with the lectin. This oligosaccharide conformation differs substantially from that seen in the previously determined isolectin I-octasaccharide complex. Comparison of our structurewith that of concanavalin A (ConA) suggests that the ConA binding site cannot accommodate this fucose. Conclusions: Our results explain theobservation that Viciae lectins have a higher affinity for fucosylated oligosaccharides than for unfucosylated ones, whereas the affinity of ConA for these types of oligosaccharides is similar. This explanation is testable by mutagenesis experiments. Our structure shows a large complementary surface area between the oligosaccharide and the lectin,in contrast with the recently determined structure of a complex between the carbohydrate recognition domain of a C-type mammalian lectin and an oligomannoside, where only the non-reducing terminal mannose residue interacts with the lectin.

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Documento generato il 23/09/20 alle ore 13:11:38