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Titolo:
ACTIVATION OF PTDINS(4,5)P-2-SENSITIVE PHOSPHOLIPASE-C IN RABBIT TRACHEAL EPITHELIAL-CELLS
Autore:
LIEDTKE CM;
Indirizzi:
CASE WESTERN RESERVE UNIV,DEPT PEDIAT & PHYSIOL,CTR CYST FIBROSIS,2101 ADELBERT RD CLEVELAND OH 44106 CASE WESTERN RESERVE UNIV,DEPT BIOPHYS CLEVELAND OH 44106
Titolo Testata:
The American journal of physiology
fascicolo: 2, volume: 266, anno: 1994,
parte:, 1
pagine: 30000397 - 30000405
SICI:
0002-9513(1994)266:2<30000397:AOPPIR>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADRENERGIC-RECEPTOR SUBTYPES; CYSTIC-FIBROSIS AIRWAY; 2ND MESSENGER PATHWAYS; ALPHA-2-ADRENERGIC RECEPTOR; ADENYLATE-CYCLASE; G-PROTEINS; CHLORIDE CHANNELS; ION-TRANSPORT; AGONIST; INHIBITION;
Keywords:
ALPHA-ADRENERGIC RECEPTOR; TRANSMEMBRANE SIGNALING; SIGNAL TRANSDUCTION; EPINEPHRINE; GUANABENZ; PERTUSSIS TOXIN; YOHIMBINE; CLONIDINE; INOSITOL TRISPHOSPHATE; PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
C.M. Liedtke, "ACTIVATION OF PTDINS(4,5)P-2-SENSITIVE PHOSPHOLIPASE-C IN RABBIT TRACHEAL EPITHELIAL-CELLS", The American journal of physiology, 266(2), 1994, pp. 30000397-30000405

Abstract

A role for phospholipase C hydrolysis of phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P-2] as a mechanism of alpha(2)-adrenergic signal transduction in rabbit tracheal epithelial cells (tracheocytes) was investigated in isolated cells grown in in vitro culture and prelabeled with myo-[H-3]inositol (3 mu Ci/ml) for 72 h. Breakdown of polyphosphoinositides was measured by using thin-layer chromatography to detectphosphatidylinositol, phosphatidylinostol 4-phosphate [PtdIns(4)P], and PtdIns(4,5)P-2. Inositol phosphates were separated by ion-exchange column chromatography. The endogenous catecholamine l-epinephrine and alpha(2)-adrenergic agonists clonidine and 1-(2,6-dichlorobenzylideneamino)guanidin (guanabenz) produced a rapid transient accumulation of inositol trisphosphate and inositol 4,5-bisphosphate and breakdown of [PtdIns(4)P] and PtdIns(4,5)P-2. The alpha(2)-adrenergic effects were not blocked by the beta-adrenergic antagonist DL-propranolol or by the alpha(1)-adrenergic antagonists prazosin and methylurapidil but were inhibited by pertussis toxin and blocked by yohimbine, an alpha(2)-adrenergic antagonist. The 50% effective concentration for guanabenz-stimulated inositol trisphosphate generation was right shifted from 0.3 to 0.9 mu M by yohimbine. The results provide the first demonstration of alpha(2A)-adrenergic activation of pertussis toxin-sensitive PtdIns(4,5)P-2-dependent phospholipase C in mammalian tracheocytes. The findings are consistent with previous observations on alpha(2A)-adrenergic-mediated activation of NaCl cotransport in these cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 05:16:49