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Titolo:
ENZYMATIC METHOD FOR THE SPECTROPHOTOMETRIC DETERMINATION OF ASPARTAME
Autore:
DINCKAYA E; CAGIN M; TELEFONCU A;
Indirizzi:
EGE UNIV,FAC SCI,DEPT BIOCHEM 35100 BORNOVA TURKEY
Titolo Testata:
Food chemistry
fascicolo: 1, volume: 50, anno: 1994,
pagine: 95 - 97
SICI:
0308-8146(1994)50:1<95:EMFTSD>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEIZURES; MICE;
Tipo documento:
Note
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
12
Recensione:
Indirizzi per estratti:
Citazione:
E. Dinckaya et al., "ENZYMATIC METHOD FOR THE SPECTROPHOTOMETRIC DETERMINATION OF ASPARTAME", Food chemistry, 50(1), 1994, pp. 95-97

Abstract

A sensitive spectrophotometric method for the determination of aspartame is described. In this method, alpha-chymotrypsin and alcohol oxidase were immobilized on highly porous Amberlite IRA 938 resin by ionic binding, to allow the enzymes to be used more than once. The method involves the enzymatic conversion of aspartame into formaldehyde and hydrogen peroxide by the immobilized alpha-chymotrypsin and alcohol-oxidase system. Horseradish peroxidase was used for the determination of hydrogen peroxide by the formation of a chromophore with 3-methyl-2-benzo-thiazolinone hydrazone (MBTH) and N,N-dimethylaniline (DMA). The absorbances of the sample solutions were measured at 595 nm against a blank. The calibration graph was linear in the range 0.1-1.0 mg ml(-1) ofaspartame.

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Documento generato il 19/09/20 alle ore 08:21:54