Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
ISOLATION AND SEQUENCE-ANALYSIS OF A GENE FROM THE LINEAR DNA PLASMIDPPACL-2 OF PICHIA-ACACIAE THAT SHOWS SIMILARITY TO A KILLER TOXIN GENE OF KLUYVEROMYCES-LACTIS
Autore:
BOLEN PL; EASTMAN EM; CIHAK PL; HAYMAN GT;
Indirizzi:
INT FLAVORS & FRAGRANCES INC,CTR CORP RES & DEV,1515 HIGHWAY 36 UNIONBEACH NJ 07735 USDA ARS,NATL CTR AGR UTILIZAT RES PEORIA IL 61604 LARK SEQUENCING TECHNOL INC HOUSTON TX 77024
Titolo Testata:
Yeast
fascicolo: 3, volume: 10, anno: 1994,
pagine: 403 - 414
SICI:
0749-503X(1994)10:3<403:IASOAG>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
SACCHAROMYCES-KLUYVERI; YEAST; EXPRESSION; PROTEINS; CHARACTER; IDENTIFICATION; INOSITOVORA; POLYMERASE; GELS;
Keywords:
YEAST; FUNGI; ZYMOCIN; PROMOTER; PGKL1; PGKL2; PSKL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
P.L. Bolen et al., "ISOLATION AND SEQUENCE-ANALYSIS OF A GENE FROM THE LINEAR DNA PLASMIDPPACL-2 OF PICHIA-ACACIAE THAT SHOWS SIMILARITY TO A KILLER TOXIN GENE OF KLUYVEROMYCES-LACTIS", Yeast, 10(3), 1994, pp. 403-414

Abstract

The toxin-encoding linear plasmid systems found in Pichia acaciae andKluyveromyces lactis yeasts appear to be quite similar, both in function and structural organization. By Southern hybridization, a linear plasmid of P. acaciae, pPacl-2, was found to hybridize to the second open reading frame (ORF2) of K. lactis plasmid pGKL1, known to encode the alpha and beta subunits of the K. lactis toxin. A 1.7 kbp segment ofpPacl-2 DNA was cloned, sequenced and shown to contain four regions of strong homology to four similarly oriented regions of K. lactis ORF2. This 1.7 kbp fragment also contained an ORF of 1473 bp that could encode a protein of similar to 55.8 kDa. Like the a subunit gene of K. lactis ORF2, a very hydrophobic region occurs at the N-terminus, perhaps representing a signal sequence for transport out of the cell. UnlikeK. lactis ORF2, however, the encoded polypeptide is much smaller and lacks a recognizable domain common to chitinases. The structure of a toxin that includes the translation product of this P. acaciae ORF would likely be quite different from that of the K. lactis toxin. Analysisof the upstream region of the P. acaciae ORF revealed an upstream conserved sequence identical to that found before ORFs 8 and 9 of pGKL2. A possible hairpin loop structure, as has been described for each of the four K. lactis pGKL1 ORFs, was found just upstream of the presumed start codon. The similarity of the promoter-like elements found in thelinear plasmid genes of these diverse yeasts reinforces the idea of the existence of a unique, but highly conserved, expression system for these novel plasmids. The sequence has been deposited in the GenBank data library under Accession Number U02596.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 23:59:28