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Titolo:
IDENTIFYING AMINO-ACID-RESIDUES THAT INFLUENCE PLASMA-CLEARANCE OF MURINE IGG1 FRAGMENTS BY SITE-DIRECTED MUTAGENESIS
Autore:
KIM JK; TSEN MF; GHETIE V; WARD ES;
Indirizzi:
UNIV TEXAS,SW MED CTR,CTR CANC IMMUNOBIOL,5323 HARRY HINES BLVD DALLAS TX 75235 UNIV TEXAS,SW MED CTR,CTR CANC IMMUNOBIOL DALLAS TX 75235 UNIV TEXAS,SW MED CTR,DEPT MICROBIOL DALLAS TX 75235
Titolo Testata:
European Journal of Immunology
fascicolo: 3, volume: 24, anno: 1994,
pagine: 542 - 548
SICI:
0014-2980(1994)24:3<542:IATIPO>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
AFFINITY FC RECEPTOR; MONOCLONAL-ANTIBODIES; PROTEIN-A; EFFECTOR FUNCTIONS; BINDING-SITE; MOUSE; REGION; DNA; CARBOHYDRATE; POLYMERASE;
Keywords:
IMMUNOGLOBULIN CATABOLISM; RECOMBINANT ANTIBODIES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
J.K. Kim et al., "IDENTIFYING AMINO-ACID-RESIDUES THAT INFLUENCE PLASMA-CLEARANCE OF MURINE IGG1 FRAGMENTS BY SITE-DIRECTED MUTAGENESIS", European Journal of Immunology, 24(3), 1994, pp. 542-548

Abstract

Site-directed mutagenesis has been used to change amino acid residuesof a recombinant Fc-hinge fragment derived from the murine immunoglobulin (Ig)G1 molecule, and the effects of these mutations on the pharmacokinetics of the Fc-hinge fragment have been determined. Specifically, Ile-253, His-310 and Gln-311 of the CH2. domain and His-433 and Asn-434 of the CH3 domain have been changed. In the three dimensional structure of an antibody, these amino acids art in close proximity to eachother at the CH2-CH3 domain interface. The mutated Fc-hinge fragmentshave been purified from recombinant Escherichia coli cells and their pharmacokinetic parameters determined in mice and compared with those of the wild-type Fc-hinge fragment. The results show that the site of the IgG1 molecule that controls the catabolic rate (the 'catabolic site') is located at the CH2-CH3 domain interface and overlaps with the Staphylococcal protein A binding site.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 10:01:38