Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
REGULATION OF CALBINDIN-D(28K) GENE-EXPRESSION IN RESPONSE TO ACUTE AND CHRONIC MORPHINE ADMINISTRATION
Autore:
TIRUMALAI PS; HOWELLS RD;
Indirizzi:
UNIV MED & DENT NEW JERSEY,NEW JERSEY MED SCH,DEPT BIOCHEM & MOLEC BIOL,185 S ORANGE AVE NEWARK NJ 07103 UNIV MED & DENT NEW JERSEY,NEW JERSEY MED SCH,DEPT BIOCHEM & MOLEC BIOL,185 S ORANGE AVE NEWARK NJ 07103
Titolo Testata:
Molecular brain research
fascicolo: 1-2, volume: 23, anno: 1994,
pagine: 144 - 150
SICI:
0169-328X(1994)23:1-2<144:ROCGIR>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
CALCIUM-BINDING PROTEIN; DELTA-OPIOID RECEPTOR; RAT LOCUS COERULEUS; MESSENGER-RNA; ADENYLATE-CYCLASE; NERVOUS-TISSUE; SPINAL-CORD; C-FOS; BRAIN; LOCALIZATION;
Keywords:
OPIATE; CALCIUM ION; CALCIUM BINDING PROTEIN; CEREBELLUM; DRUG ABUSE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
57
Recensione:
Indirizzi per estratti:
Citazione:
P.S. Tirumalai e R.D. Howells, "REGULATION OF CALBINDIN-D(28K) GENE-EXPRESSION IN RESPONSE TO ACUTE AND CHRONIC MORPHINE ADMINISTRATION", Molecular brain research, 23(1-2), 1994, pp. 144-150

Abstract

The effect of acute and chronic morphine administration on calbindin-D28K (calbindin) gene expression has been studied. One group of adult male rats received a single injection of morphine (10 mg/kg, s.c.) or saline and were sacrificed 1 or 4 h later. Another group was injected with escalating doses of morphine sulfate twice daily for 15 days to induce tolerance and physical dependence, Rats were sacrificed 1 h after the last injection. In a third group, the effect of naloxone-precipitated withdrawal on gene expression in morphine-addicted rats was alsoanalyzed 1 h after naloxone (1 mg/kg, i.p.). The cerebellum and remaining brain (minus the cerebellum) were removed, and total RNA was extracted and used for analysis. Calbindin mRNA levels in cerebellum were decreased to 30%-40% control at 1 and 4 h after a single morphine injection. Co-administration of the opiate antagonist, naloxone, reversed the effect of morphine. Tolerance developed to the acute effects in that levels were not altered significantly 1 h after morphine injection in chronically-treated rats. Unlike the cerebellum, calbindin mRNA in the remainder of the brain (minus the cerebellum) was unchanged 1 and 4 h following morphine administration to drug-naive rats, but was increased more than 2-fold compared to controls 1 h after morphine injection in chronically treated animals. Naloxone-precipitated withdrawal caused a small (20%) but significant decrease in calbindin mRNA in the cerebellum, with no change in the brain (minus the cerebellum). Rapid changes in calbindin gene expression in the cerebellum following morphine injection may play a role in acute opiate pharmacology, and the increase in calbindin expression in the brain (minus the cerebellum) may be an important aspect of the adaptation of the nervous system that results in decreased sensitivity to morphine following chronic administration.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 01:07:45