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Titolo:
LONG-TERM REPOPULATING ABILITY OF XENOGENEIC TRANSPLANTED HUMAN FETALLIVER HEMATOPOIETIC STEM-CELLS IN SHEEP
Autore:
ZANJANI ED; FLAKE AW; RICE H; HEDRICK M; TAVASSOLI M;
Indirizzi:
VET AFFAIRS MED CTR 151B,1000 LOCUST ST RENO NV 89511 UNIV NEVADA,SCH MED,DEPT VET AFFAIRS MED CTR,DEPT MED RENO NV 89520 UNIV CALIF SAN FRANCISCO,SCH MED SAN FRANCISCO CA 94143 UNIV MISSISSIPPI,SCH MED,DEPT VET AFFAIRS MED CTR JACKSON MS 39216
Titolo Testata:
The Journal of clinical investigation
fascicolo: 3, volume: 93, anno: 1994,
pagine: 1051 - 1055
SICI:
0021-9738(1994)93:3<1051:LRAOXT>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-BONE-MARROW; IMMUNE-DEFICIENT MICE; PROGENITOR CELLS; SELF-RENEWAL; ENGRAFTMENT; INUTERO; DIFFERENTIATION; PLURIPOTENT; IRRADIATION; COLONIES;
Keywords:
HEMATOPOIETIC STEM CELLS; HUMAN/SHEEP XENOGRAFT; IN UTERO TRANSPLANTATION; PREIMMUNE FETUS; FETAL LIVER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
E.D. Zanjani et al., "LONG-TERM REPOPULATING ABILITY OF XENOGENEIC TRANSPLANTED HUMAN FETALLIVER HEMATOPOIETIC STEM-CELLS IN SHEEP", The Journal of clinical investigation, 93(3), 1994, pp. 1051-1055

Abstract

We previously reported on the successful engraftment and long-term multilineage expression (erythroid, myeloid, lymphoid) of human fetal liver hematopoietic stem cells in sheep after transplantation in utero. That the engraftment of longterm repopulating pluripotent stem cells occurred in these animals was shown here by the fact that transplantation of human CD45(+) cells isolated from bone marrow of these chimeric animals into preimmune fetal sheep resulted in engraftment and expression of human cells. Marrow cells were obtained from three chimeric sheep at 3.2-3.6 yr after transplant. The relative percentage of human CD45(+) cells present in these marrows was 3.3+/-0.32%. A total of 29 X 10(6) CD45(+) cells were isolated by panning, pooled, and transplantedinto six preimmune sheep fetuses (4.8 x 10(6) cells/fetus). All six recipients were born alive. Hematopoietic progenitors exhibiting human karyotype were detected in marrows of two lambs soon after birth. Cells expressing human CD45 antigen were also detected in blood and marrowof both lambs. Human cell expression has been multilineage and has persisted for > 1 yr. These results demonstrate that the expression of human cells in this large animal model resulted from engraftment of long-term repopulating pluripotent human stem cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 00:53:27