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Titolo:
EFFECTS OF ALDEHYDE-MODIFIED PROTEINS ON MESANGIAL CELL-MATRIX INTERACTION
Autore:
HASEGAWA G; NAKANO K; TSUTSUMI Y; KONDO M;
Indirizzi:
KYOTO PREFECTURAL UNIV MED,DEPT INTERNAL MED 1,KAMIKYO KU KYOTO 602 JAPAN KYOTO PREFECTURAL UNIV MED,DEPT INTERNAL MED 1,KAMIKYO KU KYOTO 602 JAPAN
Titolo Testata:
Diabetes research and clinical practice
fascicolo: 1, volume: 23, anno: 1994,
pagine: 25 - 32
SICI:
0168-8227(1994)23:1<25:EOAPOM>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLYCOSYLATION END-PRODUCTS; NONENZYMATIC GLYCOSYLATION; DIABETIC COMPLICATIONS; AMINOGUANIDINE; TISSUE; KIDNEY; RAT; ALBUMINURIA; INTEGRINS; EXPANSION;
Keywords:
MESANGIAL CELL; CELL-MATRIX INTERACTION; GLYCOLALDEHYDE-MODIFIED PROTEIN; DIABETIC NEPHROPATHY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
G. Hasegawa et al., "EFFECTS OF ALDEHYDE-MODIFIED PROTEINS ON MESANGIAL CELL-MATRIX INTERACTION", Diabetes research and clinical practice, 23(1), 1994, pp. 25-32

Abstract

We examined the effect of aldehyde-modified matrix macromolecules on mesangial cell function in vitro, using incubated rat glomerular mesangial cells. Laminin and fibronectin were modified by incubation for 24h with 50 mM glycolaldehyde (GA), a highly reactive cross-linking glycation product, with or without equimolar aminoguanidine. GA-modified laminin and fibronectin caused marked inhibition of cell adhesion. Cell spreading was reduced on the GA-modified laminin. In contrast, the GA-modified fibronectin had no effect on cell spreading. The study of thymidine incorporation by mesangial cells showed that the GA-modified fibronectin had a diminished mitogenic activity against cells. The content of advanced glycation end-product (AGE), which was determined by fluorescence at 370 nm excitation and 440 nm emission wavelength, increased and intermolecular cross-links appeared in the GA-modified proteins. To a large extent, aminoguanidine restored the structural alterations and functional deteriorations described above. We conclude that GA-modified matrix proteins diminish their functional properties against mesangial cells and affect cellular functions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 21:31:29