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Titolo:
A RADIATION HYBRID MAP OF HUMAN CHROMOSOME-18
Autore:
FRANCKE U; CHANG E; COMEAU K; GEIGL EM; GIACALONE J; LI X; LUNA J; MOON A; WELCH S; WILGENBUS P;
Indirizzi:
STANFORD UNIV,MED CTR,SCH MED,HOWARD HUGHES MED INST STANFORD CA 94305 STANFORD UNIV,MED CTR,SCH MED,DEPT GENET STANFORD CA 94305
Titolo Testata:
Cytogenetics and cell genetics
fascicolo: 3, volume: 66, anno: 1994,
pagine: 196 - 213
SICI:
0301-0171(1994)66:3<196:ARHMOH>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; HUMAN X-CHROMOSOME; ALU-PCR PRODUCTS; INSITU HYBRIDIZATION; WILLIAMS SYNDROME; RAPID ISOLATION; HUMAN GENOME; LINKAGE MAP; LONG ARM; LOCI;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
53
Recensione:
Indirizzi per estratti:
Citazione:
U. Francke et al., "A RADIATION HYBRID MAP OF HUMAN CHROMOSOME-18", Cytogenetics and cell genetics, 66(3), 1994, pp. 196-213

Abstract

A human x Chinese hamster (CH) somatic cell hybrid subclone deficientin HPRT and containing only human chromosome 18 was irradiated with 7000 rad and fused to a thymidine kinase deficient CH cell line. Radiation-rescued hybrid cell lines, selected in HAT medium, were analyzed for human DNA with human interspersed-repeat sequence primers. Size andnumber of human chromosome fragments retained in a subset of hybrids were determined by FISH. A panel of 98 radiation hybrids (RH) was selected and analyzed for 90 chromosome 18-specific STSs by PCR, and for the D18Z1 centromeric marker by Southern blotting. STSs were developed from previously mapped RFLP loci and from published sequences. In addition, 32 novel STSs were generated from an 18-specific lambda library and from 18-specific YACs previously localized to chromosome bands by FISH, Marker retention frequency varied from 8-65 % with an average of24 %. In selected RH the STS typing data were correlated with the chromosome 18 regions retained using 'reverse FISH' of IRS-PCR products from the RH to normal metaphase chromosomes. The order and intermarker distances of loci were determined using two-point and multipoint maximum likelihood methods. The resulting RH map covers most of chromosome 18 with four groups of tightly linked markers and three regions of loosely linked markers, one around the centromere and two on the long arm. More than a third of the markers are polymorphic and allow integration with the linkage map. This RH map provides a framework for establishing a clone contig of the entire chromosome 18.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 15:54:48