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Titolo:
CHARACTERIZATION OF A SPECIES OF NONSPECIFIC CROSS-REACTING ANTIGEN (NCA) EXPRESSED BY HUMAN MONOCYTIC CELL-LINES - STRUCTURE AND EXPRESSION DURING CELL-DIFFERENTIATION
Autore:
KUROKI M; YAMANAKA T; MATSUO Y; OHTANI T; MATSUO Y; MINOWADA J; MISUMI Y; OIKAWA S; NAKAZATO H; MATSUOKA Y;
Indirizzi:
FUKUOKA UNIV,SCH MED,DEPT BIOCHEM,JONAN KU,7-45-1 NANAKUMA FUKUOKA 81401 JAPAN FUKUOKA UNIV,SCH MED,DEPT BIOCHEM,JONAN KU FUKUOKA 81401 JAPAN HAYASHIBARA BIOCHEM LABS INC,FUJISAKI CELL CTR OKAYAMA JAPAN SUNTORY INST BIOMED RES OSAKA JAPAN
Titolo Testata:
International journal of cancer
fascicolo: 6, volume: 56, anno: 1994,
pagine: 886 - 891
SICI:
0020-7136(1994)56:6<886:COASON>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
CEA GENE FAMILY; MONOCLONAL-ANTIBODIES; ADHESION ACTIVITY; SURFACE; MEMBERS; GRANULOCYTES; SEQUENCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
M. Kuroki et al., "CHARACTERIZATION OF A SPECIES OF NONSPECIFIC CROSS-REACTING ANTIGEN (NCA) EXPRESSED BY HUMAN MONOCYTIC CELL-LINES - STRUCTURE AND EXPRESSION DURING CELL-DIFFERENTIATION", International journal of cancer, 56(6), 1994, pp. 886-891

Abstract

It has been documented that human monocytes/macrophages are reactive with antibodies directed to carcinoembryonic antigen (CEA) and non-specific cross-reacting antigens (NCAs), a group of glycoproteins antigenically cross-reactive with CEA, yet the molecules responsible for thisantigenic activity have not been fully clarified. In the present study, among 7 myelomonocytic cell lines tested, 2 monoblastoid lines, U-937 and THP-1, were found to express NCA-50/90, a glycosyl-phosphatidylinositol-anchored cell-adhesion molecule chiefly expressed on granulocytes. The 2 cell lines showed a reaction pattern with 5 distinct anti-CEA and anti-NCA monoclonal antibodies, similar to that of CHO transfectants expressing recombinant NCA-50/90. Immunoprecipitation and SDS-PAGE analyses identified glycoproteins of about 95 and 55 kDa in U-937 and THP-1 cells, respectively. Deglycosylation of the 2 antigens with N-glycanase gave the same apparent molecular mass of about 45,000, which was also the same as that of the deglycosylated form of the recombinant NCA-50/90. Upon Northern-blot analysis, only one band of approximately 2.5 kb was detected in both cell lines with a cDNA probe for NCA-50/90, which has a broad specificity to the CEA gene family members. cDNA cloning demonstrated that the 2.5-kb clones encode the peptide ofNCA-50/90. The expression of NCA-50/90 by U-937 and THP-1 was down-regulated at both the protein and mRNA levels during cell differentiation from monoblastoid to monocyte/macrophage-like cells induced by stimulation with phorbol 12-myristate 13-acetate. Our observations suggest that NCA-50/90 is a differentiation antigen of cells of the monocyte/macrophage lineage as well as of the granulocyte lineage. (C) 1994 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 16:06:31