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Titolo:
EXPRESSION AND FUNCTION OF THE INSULIN-LIKE GROWTH-FACTOR-I SYSTEM INHUMAN NON-SMALL-CELL LUNG-CANCER AND NORMAL LUNG-CELL LINES
Autore:
FAVONI RE; DECUPIS A; RAVERA F; CANTONI C; PIRANI P; ARDIZZONI A; NOONAN D; BIASSONI R;
Indirizzi:
IST NAZL RIC CANC,DEPT EXPTL PHARMACOL,VIALE BENEDETTO XV 10 I-16132 GENOA ITALY IST NAZL RIC CANC,DEPT MED ONCOL 1 I-16132 GENOA ITALY IST NAZL RIC CANC,DEPT CHEM CARCINOGENESIS I-16132 GENOA ITALY
Titolo Testata:
International journal of cancer
fascicolo: 6, volume: 56, anno: 1994,
pagine: 858 - 866
SICI:
0020-7136(1994)56:6<858:EAFOTI>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
BINDING-PROTEINS; IGF-I; RECEPTOR; TUMORS; CULTURE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
R.E. Favoni et al., "EXPRESSION AND FUNCTION OF THE INSULIN-LIKE GROWTH-FACTOR-I SYSTEM INHUMAN NON-SMALL-CELL LUNG-CANCER AND NORMAL LUNG-CELL LINES", International journal of cancer, 56(6), 1994, pp. 858-866

Abstract

In order to analyze the presence and the function of the ''insulin-like growth factor I (IGF-I) system'' in human non-small-cell lung cancer (N-SCLC) we tested 5 cell lines of different histological sub-types:A549, Ca-Lu-6, SK-Lu-1 (adenocarcinoma); Ca-Lu-1, SK-Mes-1 (squamous carcinoma) and one normal fibroblast-like fetal lung cell line (IMR-90) for expression of the IGF-I peptide and its RNA transcribed from theIGF-I gene; IGF-binding proteins (IGF-BP); IGF-I receptor (IGF-I-R) and its mRNA. In addition, we examined the capacity of exogenous human recombinant IGF-I to enhance the in vitro cell proliferation. In medium conditioned from cell cultures, we detected immunoreactive IGF-I material by radioimmunoassay. Western ligand blot and affinity labelling demonstrated the presence of several molecular species of IGF-BPs (IGF-BP-4, -1, -2, -3) as well. Northern blot analysis of polyA(+) RNA from all cell lines examined revealed the presence of IGF-I and IGF-I-R mRNA. Moreover, binding studies on cultured cell lines showed one classof high-affinity, operative type-I IGF cell-surface binding sites. Finally, by thymidine uptake and colorimetric metabolic MTT assays, we found that most neoplastic cell lines react mitogenically to IGF-I and that its physiological effect is abolished by an anti-IGF-I-receptor antibody. These data indicate the importance of the IGF-I system in N-SCLC growth. Furthermore, they suggest that this mitogenic complex should be appraised as a possible target for anti-neoplastic drugs, antibodies or growth-factor analogues offering potential new approaches to therapy. (C) 1994 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 11:36:10