Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
XENOPUS-LAEVIS OVARIAN DNA HELICASE .1. A 3' TO 5' HELICASE THAT UNWINDS SHORT DUPLEXES
Autore:
POLL EHA; HARRISON J; UMTHUN A; DOBBS DL; BENBOW RM;
Indirizzi:
IOWA STATE UNIV SCI & TECHNOL,NUCLEIC ACID RES FACIL,2258 MOLEC BIOL BLDG AMES IA 50011 IOWA STATE UNIV SCI & TECHNOL,NUCLEIC ACID RES FACIL,2258 MOLEC BIOL BLDG AMES IA 50011 IOWA STATE UNIV SCI & TECHNOL,DEPT ZOOL AMES IA 50011 IOWA STATE UNIV SCI & TECHNOL,GENET MOLEC CELLULAR & DEV BIOL PROGRAMAMES IA 50011 IOWA STATE UNIV SCI & TECHNOL,INTERDEPARTMENTAL GENET PROGRAM AMES IA50011
Titolo Testata:
Biochemistry
fascicolo: 13, volume: 33, anno: 1994,
pagine: 3841 - 3847
SICI:
0006-2960(1994)33:13<3841:XODH.A>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOUSE FM3A CELLS; DEPENDENT ADENOSINETRIPHOSPHATASE-B; SINGLE-STRANDED-DNA; LARGE TUMOR-ANTIGEN; LARGE T-ANTIGEN; CALF THYMUS; HELA-CELLS; SACCHAROMYCES-CEREVISIAE; PROTEIN; PURIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
E.H.A. Poll et al., "XENOPUS-LAEVIS OVARIAN DNA HELICASE .1. A 3' TO 5' HELICASE THAT UNWINDS SHORT DUPLEXES", Biochemistry, 33(13), 1994, pp. 3841-3847

Abstract

A novel DNA helicase isolated from Xenopus laevis ovaries [Poll, E. H. A.. & Benbow, R.M. (1988) Biochemistry 27, 8701-8706] was characterized biochemically. The directionality of DNA unwinding was determined to be 3' to 5'. A short 3' ssDNA tail adjacent to duplex DNA was required for DNA unwinding; the minimum length of this tail was between four and nine bases. Only short duplex DNA regions were unwound: duplex DNA of 16 base pairs was readily unwound, whereas a 26 base pair duplexwas not. Longer duplex regions were unwound in the presence of Escherichia coli single-strand DNA binding protein if, in addition, the duplex region was flanked by an unpaired 3' or 5' tail and the substrate resembled a branched replicative intermediate. X. laevis DNA helicase Iexhibited high affinity for ssDNA, moderate affinity for dsDNA, and no affinity for RNA. DNA unwinding activity was stimulated by monovalent cations, with an optimal concentration of 150 mM for NaCl or KCl or 125 mM for NaxPO4 or KxPO4. The ATP analog ATPgammaS inhibited the DNAunwinding and copurifying DNA-dependent ATPase activity, whereas AMPPCP and AMPPNP moderately inhibited DNA unwinding activity and had little effect on the copurifying DNA-dependent ATPase activity. CTP was a relatively strong inhibitor of DNA unwinding activity, but GTP, UTP, dCTP, dGTP, or TTP showed moderate or no inhibition. The copurifying DNA-dependent ATPase activity was not inhibited by CTP, GTP, UTP, dCTP, dGTP, or TTP.

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Documento generato il 01/10/20 alle ore 16:16:40