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Titolo:
CHANGES OF COLON EPITHELIUM PROLIFERATION DUE TO INDIVIDUAL AGING WITH CYCLIN PROLIFERATING CELL NUCLEAR ANTIGEN (PCNA CYCLIN) IMMUNOSTAINING COMPARED TO [H-3] THYMIDINE AUTORADIOGRAPHY
Autore:
MORITA T; USUDA N; HANAI T; NAGATA T;
Indirizzi:
SHINSHU UNIV,DEPT ANAT & CELL BIOL,3-1-1 ASAHI MATSUMOTO NAGANO 390 JAPAN
Titolo Testata:
Histochemistry
fascicolo: 1, volume: 101, anno: 1994,
pagine: 13 - 20
SICI:
0301-5564(1994)101:1<13:COCEPD>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA POLYMERASE-DELTA; MONOCLONAL-ANTIBODIES; DESCENDING COLON; S-PHASE; PROTEIN; MOUSE; REPLICATION; AUTORADIOGRAPHY; ARGENTAFFIN; PHOSPHATASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
T. Morita et al., "CHANGES OF COLON EPITHELIUM PROLIFERATION DUE TO INDIVIDUAL AGING WITH CYCLIN PROLIFERATING CELL NUCLEAR ANTIGEN (PCNA CYCLIN) IMMUNOSTAINING COMPARED TO [H-3] THYMIDINE AUTORADIOGRAPHY", Histochemistry, 101(1), 1994, pp. 13-20

Abstract

We report a change in the proliferative activity of mouse colonic epithelium due to development and aging. In order to measure the proliferative activity, colonic epithelium was immunostained for cyclin proliferating cell nuclear antigen (PCNA/cyclin), which appears from the GI to the S phase of the cell cycle, and compared with labeling obtained by [H-3]-thymidine radioautography. Litter mice of six age groups fromthe fetal period (embryonic day 19), newborn period (postnatal day 1), suckling period (postnatal day 5), weaning period (postnatal dy 21),adult period (2 month old) to the senescent period (11 month old) were examined by immunohistochemistry. The descending colons were fixed in methacarn (method-Carnoy) and embedded in paraffin. Sections were stained for PCNA/cyclin activity using 19A2 monoclonal antibody and the avidin-biotin peroxidase complex (ABC) technique. For radioautography,litter mice of nine age groups using in vivo intraperitoneal administration of [H-3]-thymidine. The labeling indices of colonic epithelial cells in the proliferative zone were then analyzed and compared between the two investigative methods. Our results show that the proliferative activity of mice colon was high in the fetal and newborn periods and almost constant from the suckling period to senescence, as demonstrated by both PCNA/cyclin immunohistochemistry and [H-3]thymidine radioautography. The labeling index seen by PCNA/cyclin immunohistochemistrywas, however, higher than that seen by [H-3]-thymidine radioautography.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 10:03:15