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Titolo:
A PHOSPHOROTHIOATE OLIGONUCLEOTIDE BLOCKS REVERSE TRANSCRIPTION VIA AN ANTISENSE MECHANISM
Autore:
BOIZIAU C; MOREAU S; TOULME JJ;
Indirizzi:
UNIV BORDEAUX 2,INSERM,U386,BIOPHYS MOLEC LAB,146 RUE LEO SAIGNAT F-33076 BORDEAUX FRANCE UNIV BORDEAUX 2,INSERM,U386,BIOPHYS MOLEC LAB F-33076 BORDEAUX FRANCE
Titolo Testata:
FEBS letters
fascicolo: 3, volume: 340, anno: 1994,
pagine: 236 - 240
SICI:
0014-5793(1994)340:3<236:APOBRT>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; CHRONICALLY INFECTED-CELLS; GENE-EXPRESSION; RNA-POLYMERASE; NUCLEIC-ACIDS; INHIBITION; OLIGODEOXYNUCLEOTIDES; INVITRO; DNA; REPLICATION;
Keywords:
ANTISENSE OLIGONUCLEOTIDE; INHIBITION MECHANISM; RETROVIRUS; RNASE H;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
C. Boiziau et al., "A PHOSPHOROTHIOATE OLIGONUCLEOTIDE BLOCKS REVERSE TRANSCRIPTION VIA AN ANTISENSE MECHANISM", FEBS letters, 340(3), 1994, pp. 236-240

Abstract

We have studied the inhibition by a phosphorothioate oligodeoxynucleotide (17PScap) of cDNA synthesis performed by either avian or murine reverse transcriptase, Three different mechanisms of inhibition were identified: at low concentrations (<100 nM), the cleavage of the RNA template by the retroviral RNase H at the level of the RNA/17PScap duplexaccounted for most of the effect, whereas hybrid-arrested cDNA synthesis by an RNase H-independent mechanism marginally contributed to the inhibition. Both mechanisms were sequence-specific. Above 100 nM, the overall cDNA synthesis was reduced in a non-specific manner.

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Documento generato il 27/09/20 alle ore 00:56:01