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Titolo:
OVEREXPRESSION OF NEF AS A MARKER FOR RESTRICTED HIV-1 INFECTION OF ASTROCYTES IN POSTMORTEM PEDIATRIC CENTRAL NERVOUS TISSUES
Autore:
SAITO Y; SHARER LR; EPSTEIN LG; MICHAELS J; MINTZ M; LOUDER M; GOLDING K; CVETKOVICH TA; BLUMBERG BM;
Indirizzi:
UNIV ROCHESTER,MED CTR,DEPT NEUROL,MOLEC NEUROVIROL LAB,BOX 605,601 ELMWOOD AVE ROCHESTER NY 14642 UNIV ROCHESTER,MED CTR,DEPT NEUROL,MOLEC NEUROVIROL LAB,BOX 605,601 ELMWOOD AVE ROCHESTER NY 14642 UNIV ROCHESTER,MED CTR,DEPT MICROBIOL & IMMUNOL ROCHESTER NY 14642 UNIV ROCHESTER,MED CTR,DEPT PEDIAT ROCHESTER NY 14642 UNIV MED & DENT NEW JERSEY,NEW JERSEY MED SCH,DEPT NEUROSCI NEWARK NJ07103 UNIV MED & DENT NEW JERSEY,NEW JERSEY MED SCH,DEPT LAB MED & PATHOL NEWARK NJ 07103
Titolo Testata:
Neurology
fascicolo: 3, volume: 44, anno: 1994,
pagine: 474 - 481
SICI:
0028-3878(1994)44:3<474:OONAAM>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; IMMUNE-DEFICIENCY SYNDROME; TUMOR-NECROSIS-FACTOR; BRAIN-TISSUE; AIDS ENCEPHALOPATHY; III INFECTION; GLIAL-CELLS; HTLV-III; PROGRESSIVE ENCEPHALOPATHY; CELLULAR-LOCALIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
72
Recensione:
Indirizzi per estratti:
Citazione:
Y. Saito et al., "OVEREXPRESSION OF NEF AS A MARKER FOR RESTRICTED HIV-1 INFECTION OF ASTROCYTES IN POSTMORTEM PEDIATRIC CENTRAL NERVOUS TISSUES", Neurology, 44(3), 1994, pp. 474-481

Abstract

In previous studies, using polymerase chain reaction amplification ofHIV-1 genes directly from pathologic tissues of children who died with AIDS encephalopathy, we showed that the reading frame of the. HIV-1 regulatory nef gene is open, suggesting that the nef protein was expressed. We now show, using immunocytochemistry and in situ hybridizationwith nef-specific probes in postmortem pediatric CNS tissues, that nef mRNA and protein are present in up to 20% of astrocytes in tissue sections selected for extensive histopathology. By contrast, HIV-1 structural proteins such as gag and their coding mRNAs are present in multinucleated giant cells that harbor productive infection and are the hallmark of HIVV-1 infection in the CNS. These findings are consistent with the nonproductive infection of glial cells observed in vitro, and imply that HIV-1 infection of astrocytes is restricted to early regulatory gene products, of which nef is the best target as it is expressed at high levels; and is membrane-anchored. In developing central nervous tissues of children, restricted and latent HrV-1 infection of astrocytes may be extensive and contribute significantly to HIV-1 neuropathogenesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 18:19:36