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Titolo:
GENETICS OF THE SERINE CYCLE IN METHYLOBACTERIUM-EXTORQUENS AM1 - IDENTIFICATION OF SGAA AND MTDA AND SEQUENCES OF SGAA, HPRA, AND MTDA
Autore:
CHISTOSERDOVA LV; LIDSTROM ME;
Indirizzi:
CALTECH,WM KECK LABS 13878 PASADENA CA 91125 CALTECH,WM KECK LABS 13878 PASADENA CA 91125
Titolo Testata:
Journal of bacteriology
fascicolo: 7, volume: 176, anno: 1994,
pagine: 1957 - 1968
SICI:
0021-9193(1994)176:7<1957:GOTSCI>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEHYDROGENASE-METHENYLTETRAHYDROFOLATE CYCLOHYDROLASE; D-LACTATE DEHYDROGENASE; AMINO-ACID-SEQUENCE; NUCLEOTIDE-SEQUENCE; METHYLENETETRAHYDROFOLATE DEHYDROGENASE; HYDROXYPYRUVATE REDUCTASE; ESCHERICHIA-COLI; FORMYLTETRAHYDROFOLATE SYNTHETASE; C1-TETRAHYDROFOLATE SYNTHASE; GLYOXYLATE AMINOTRANSFERASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
63
Recensione:
Indirizzi per estratti:
Citazione:
L.V. Chistoserdova e M.E. Lidstrom, "GENETICS OF THE SERINE CYCLE IN METHYLOBACTERIUM-EXTORQUENS AM1 - IDENTIFICATION OF SGAA AND MTDA AND SEQUENCES OF SGAA, HPRA, AND MTDA", Journal of bacteriology, 176(7), 1994, pp. 1957-1968

Abstract

In a previous paper, we reported identification of the 5' part of hprA of Methylobacterium extorquens AM1, which encodes the serine cycle enzyme hydroxypyruvate reductase (L. V. Chistoserdova and M. E. Lidstrom, J. Bacteriol. 174:71-77, 1992). Here we present the complete sequence of hprA and partial sequence of genes adjacent to hprA. Upstream ofhprA, the 3' part of an open reading frame was discovered, separated from hprA by 263 bp. This open reading frame was identified as the gene encoding another serine cycle enzyme, serine glyoxylate aminotransferase (sgaA). Cells containing an insertion mutation into sgaA were unable to grow on C1 compounds, demonstrating that the gene is required for C1 metabolism. Sequencing downstream of hprA has revealed the presence of another open reading frame (mtdA), which is probably cotranscribed with hprA. This open reading frame was identified as the gene required for the synthesis of 5,10-methylenetetrahydrofolate dehydrogenase. Our data suggest that this enzyme plays an integral role in methylotrophic metabolism in M. extorquens AM1, either in formaldehyde oxidation or as part of the serine cycle.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 15:47:38