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Titolo:
DIFFERENTIAL REGULATION OF CYCLIC ADENOSINE-3',5'-MONOPHOSPHATE (CAMP) RESPONSE ELEMENT-BINDING PROTEIN AND CAMP RESPONSE ELEMENT MODULATORMESSENGER-RIBONUCLEIC-ACID TRANSCRIPTS BY FOLLICLE-STIMULATING-HORMONE AND ANDROGEN IN THE ADULT-RAT TESTIS
Autore:
WEST AP; SHARPE RM; SAUNDERS PTK;
Indirizzi:
MRC,CTR REPROD BIOL,REPROD BIOL UNIT,37 CHALMERS ST EDINBURGH EH3 9EWMIDLOTHIAN SCOTLAND
Titolo Testata:
Biology of reproduction
fascicolo: 4, volume: 50, anno: 1994,
pagine: 869 - 881
SICI:
0006-3363(1994)50:4<869:DROCA(>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEMINIFEROUS EPITHELIAL CYCLE; CELL-INTERACTIONS; SERTOLI CELLS; SPERMATOGENESIS; TESTOSTERONE; RECEPTOR; RNA; DNA; GENERATION; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
A.P. West et al., "DIFFERENTIAL REGULATION OF CYCLIC ADENOSINE-3',5'-MONOPHOSPHATE (CAMP) RESPONSE ELEMENT-BINDING PROTEIN AND CAMP RESPONSE ELEMENT MODULATORMESSENGER-RIBONUCLEIC-ACID TRANSCRIPTS BY FOLLICLE-STIMULATING-HORMONE AND ANDROGEN IN THE ADULT-RAT TESTIS", Biology of reproduction, 50(4), 1994, pp. 869-881

Abstract

Hormonal regulation of the expression of mRNA transcripts for cAMP response element-binding protein (CREB) and cAMP response element modulator (CREM) during spermatogenesis was studied in the adult rat testis. Northern analysis of CREB and CREM identified two mRNA transcripts for CREM (2.4 and 1.6 kb) and one transcript for CREB (2.0 kb). Analysisof mRNAs from isolated testicular cells by reverse transcriptase polymerase chain reaction (RT/PCR) showed that CREM mRNAs were expressed by the germ cells but not the Sertoli or interstitial cells, whereas CREB mRNA was located in germ cells, Sertoli cells, and interstitial cells. RNA was isolated and analyzed from the testes of 1) rats treated for 24 h with FSH, 2) rats in which androgen withdrawal had been induced by ethane dimethane sulphonate (EDS) treatment 6 days earlier (EDS-treated), 3) EDS-treated rats supplemented with testosterone (EDS + T),or 4) intratesticular administration of dibutyryl cAMP (dbcAMP) in the preceding 24 h. CREM mRNA transcript expression was found to be decreased after all of these treatments in samples from intact testis and from isolated cells. Expression of the CREB transcript was also decreased by EDS-induced androgen withdrawal, but not by FSH or EDS + T. In situ hybridization of paraffin-embedded testis sections probed with digoxigenin-labeled riboprobes confirmed the localization of CREB and CREM mRNA to the same cell types as found with RT/PCR. No stage-dependent expression of CREM mRNA transcripts could be observed. Hybridizationof the CREB probe was highest around the base of stage VII-VIII tubules, and this was shown to be androgen-dependent. The data presented suggest that regulation of the expression of CRE-binding protein mRNAs in Sertoli and germ cells during spermatogenesis is dependent on both androgen and FSH. However, the effects of androgen or FSH on the regulation of CRE-binding protein mRNAs are different.

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Documento generato il 11/07/20 alle ore 04:43:02