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Titolo:
CHARACTERIZATION OF MUSCARINIC ACETYLCHOLINE-RECEPTORS EXPRESSED BY AN ATRIAL CELL-LINE DERIVED FROM A TRANSGENIC MOUSE-TUMOR
Autore:
MORTON ME; BRUMWELL C; GARTSIDE CL; HAUSCHKA SD; NATHANSON NM;
Indirizzi:
UNIV WASHINGTON,DEPT PHARMACOL,SJ-30 SEATTLE WA 98195 UNIV WASHINGTON,DEPT PHARMACOL,SJ-30 SEATTLE WA 98195 UNIV WASHINGTON,DEPT BIOCHEM SEATTLE WA 98195 COLL HOLY CROSS,DEPT BIOL WORCESTER MA 01610
Titolo Testata:
Circulation research
fascicolo: 4, volume: 74, anno: 1994,
pagine: 752 - 756
SICI:
0009-7330(1994)74:4<752:COMAEB>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOLECULAR-CLONING; MESSENGER-RNAS; CHICK HEART; RAT-HEART; T-ANTIGEN; BINDING; PROTEIN; MICE; SUBUNIT; INVIVO;
Keywords:
G-PROTEINS; PHOSPHOLIPASE-C; ADENYLYL CYCLASE; CARDIAC CELL CULTURES; SV40 T-ANTIGEN;
Tipo documento:
Note
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
M.E. Morton et al., "CHARACTERIZATION OF MUSCARINIC ACETYLCHOLINE-RECEPTORS EXPRESSED BY AN ATRIAL CELL-LINE DERIVED FROM A TRANSGENIC MOUSE-TUMOR", Circulation research, 74(4), 1994, pp. 752-756

Abstract

The properties of muscarinic acetylcholine receptors in the cell lineMCM1, derived from an SV40 T-antigen-induced atrial tumor in a transgenic mouse, were determined. Binding studies using the nonselective muscarinic antagonist [H-3]quinuclidinyl benzilate, the M1-selective antagonist pirenzepine, and the M2-selective antagonist AFDX-116 indicatethat the receptors have the pharmacological properties of the cardiac(M2) receptor subtype. The receptors could be immunoprecipitated witha monoclonal antibody specific for the cardiac receptor, thus confirming the identity of the receptors expressed in these cells. The types of G proteins expressed in the cells were determined by Northern blot analyses: mRNA encoding the alpha subunits of G(s), G(o), and G(i-2), but not G(i-1) or G(i-3), were detected, consistent with previous observations of neonatal mammalian atria. The muscarinic receptors were functionally active, as demonstrated by the ability of the agonist to stimulate phosphoinositide turnover and to inhibit adenylyl cyclase activity. The availability of a mammalian atrial cell line that continues to express the appropriate functionally coupled subtype of muscarinic receptor may provide a useful system for the investigation of the regulation of expression and function of cardiac muscarinic receptors.

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Documento generato il 30/10/20 alle ore 08:45:18