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Titolo:
HIS-154 IS INVOLVED IN THE LINKAGE OF THE SACCHAROMYCES-CEREVISIAE L-A DOUBLE-STRANDED-RNA VIRUS GAG PROTEIN TO THE CAP STRUCTURE OF MESSENGER-RNAS AND IS ESSENTIAL FOR M(1) SATELLITE VIRUS EXPRESSION
Autore:
BLANC A; RIBAS JC; WICKNER RB; SONENBERG N;
Indirizzi:
MCGILL UNIV,DEPT BIOCHEM,MCINTYRE MED BLDG,3655 DRUMMOND ST,ROOM 807 MONTREAL H3G 1Y6 QUEBEC CANADA MCGILL UNIV,DEPT BIOCHEM,MCINTYRE MED BLDG,3655 DRUMMOND ST,ROOM 807 MONTREAL H3G 1Y6 QUEBEC CANADA MCGILL UNIV,MCGILL CANC CTR MONTREAL H3G 1Y6 QUEBEC CANADA NIDDKD,BIOCHEM PHARMACOL LAB,GENET SIMPLE EUKARYOTES SECT BETHESDA MD20892
Titolo Testata:
Molecular and cellular biology
fascicolo: 4, volume: 14, anno: 1994,
pagine: 2664 - 2674
SICI:
0270-7306(1994)14:4<2664:HIIITL>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
HELA NUCLEAR EXTRACT; YEAST KILLER TOXIN; POL FUSION PROTEIN; BINDING-PROTEINS; SPLICING INVITRO; CYTO-PATHOLOGY; COAT PROTEIN; REPLICATION; INITIATION; POLYMERASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
64
Recensione:
Indirizzi per estratti:
Citazione:
A. Blanc et al., "HIS-154 IS INVOLVED IN THE LINKAGE OF THE SACCHAROMYCES-CEREVISIAE L-A DOUBLE-STRANDED-RNA VIRUS GAG PROTEIN TO THE CAP STRUCTURE OF MESSENGER-RNAS AND IS ESSENTIAL FOR M(1) SATELLITE VIRUS EXPRESSION", Molecular and cellular biology, 14(4), 1994, pp. 2664-2674

Abstract

The coat protein (Gag) of the double-stranded RNA virus L-A was previously shown to form a covalent bond with the cap structure of eukaryotic mRNAs. Here, we identify the linkage as a phosphoroimidazole bond between the alpha phosphate of the cap structure and a nitrogen in the Gag protein His-154 imidazole side chain. Mutations of His-154 abrogate the ability of Gag to bind to the cap structure, without affecting cap recognition, in vivo virus particle formation from an L-A cDNA clone, or in vitro specific binding and replication of plus-stranded single-stranded RNA. However, genetic analyses demonstrate that His-154 is essential for M1 satellite virus expression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 18:36:28