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Titolo:
CHARACTERIZATION OF CIS-ACTING ELEMENTS IN LIGHT REGULATION OF THE NUCLEAR GENE ENCODING THE A-SUBUNIT OF CHLOROPLAST ISOZYMES OF GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE FROM ARABIDOPSIS-THALIANA
Autore:
CONLEY TR; PARK SC; KWON HB; PENG HP; SHIH MC;
Indirizzi:
UNIV IOWA,DEPT BIOL SCI IOWA CITY IA 52242 UNIV IOWA,DEPT BIOL SCI IOWA CITY IA 52242
Titolo Testata:
Molecular and cellular biology
fascicolo: 4, volume: 14, anno: 1994,
pagine: 2525 - 2533
SICI:
0270-7306(1994)14:4<2525:COCEIL>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; CARBOXYLASE SMALL SUBUNIT; MESSENGER-RNA; HIGHER-PLANTS; RBCS-3A GENE; EXPRESSION; PHYTOCHROME; PROMOTER; BINDING; DNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
T.R. Conley et al., "CHARACTERIZATION OF CIS-ACTING ELEMENTS IN LIGHT REGULATION OF THE NUCLEAR GENE ENCODING THE A-SUBUNIT OF CHLOROPLAST ISOZYMES OF GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE FROM ARABIDOPSIS-THALIANA", Molecular and cellular biology, 14(4), 1994, pp. 2525-2533

Abstract

We have characterized cis-acting elements involved in light regulation of the nuclear gene (GapA) encoding the A subunit of chloroplast glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in Arabidopsis thaliana. Our results show that a 1.1-kb promoter fragment of the GapA gene is sufficient to confer light inducibility and organ specificity in transgenic Nicotiana tabacum (tobacco) plants, using the beta-glucuronidasegene of Escherichia coli as the reporter gene. Deletion analysis indicates that the -359 to -110 bp region of the GapA gene is necessary for light responsiveness. Within this region there are three copies of adecamer repeat (termed the Gap box) having the consensus sequence 5'-CAAATGAA(A/G)A-3', which has not been characterized in the promoter regions of other light-regulated genes. A deletion (to -247) producing loss of one copy of these elements from the GapA promoter reduces lightinduction by two- to threefold compared with a promoter deletion (to -359) with all three Gap boxes present, while deletion of all three Gap boxes (to -110) abolishes light induction completely. Gel mobility shift experiments using tobacco nuclei as the source of nuclear proteins show that GapA promoter fragments that contain these repeats bind strongly to a factor in the nuclear extract and that binding can be abolished by synthetic competitors consisting only of a monomer or dimer of the Gap box. Furthermore, a trimer, dimer, and monomer of the Gap box show binding activity and, like the authentic GapA promoter-derived probes, show binding activities that are correlated with Gap box copy number. These results strongly suggest that these repeats play important roles in light regulation of the GapA gene of A. thaliana.

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Documento generato il 30/11/20 alle ore 00:13:10