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Titolo:
METABOLIC AND BIOSYNTHETIC ALTERATIONS IN CULTURED ASTROCYTES EXPOSEDTO HYPOXIA REOXYGENATION
Autore:
HORI O; MATSUMOTO M; MAEDA Y; UEDA H; OHTSUKI T; STERN DM; KINOSHITA T; OGAWA S; KAMADA T;
Indirizzi:
OSAKA UNIV,MED SCH HOSP,DEPT MED 1,2-2 YAMADA OKA SUITA OSAKA 565 JAPAN OSAKA UNIV,MED SCH HOSP,DEPT MED 1,2-2 YAMADA OKA SUITA OSAKA 565 JAPAN OSAKA UNIV,MED SCH HOSP,DEPT NEUROL SUITA OSAKA 565 JAPAN OSAKA UNIV,MICROBIAL DIS RES INST,DEPT IMMUNOREGULAT SUITA OSAKA 565 JAPAN COLUMBIA UNIV COLL PHYS & SURG,DEPT PHYSIOL & CELLULAR BIOPHYS NEW YORK NY 10032
Titolo Testata:
Journal of neurochemistry
fascicolo: 4, volume: 62, anno: 1994,
pagine: 1489 - 1495
SICI:
0022-3042(1994)62:4<1489:MABAIC>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUPEROXIDE-DISMUTASE; ENDOTHELIAL-CELLS; RAT-BRAIN; INJURY; ISCHEMIA; PROTEINS; GERBILS; GROWTH; TISSUE; DAMAGE;
Keywords:
GLIA; ENERGY METABOLISM; NEUROTROPHIC FUNCTION; STRESS RESPONSE; NADPH OXIDASE; REPERFUSION INJURY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
O. Hori et al., "METABOLIC AND BIOSYNTHETIC ALTERATIONS IN CULTURED ASTROCYTES EXPOSEDTO HYPOXIA REOXYGENATION", Journal of neurochemistry, 62(4), 1994, pp. 1489-1495

Abstract

To investigate the astrocyte response to hypoxia/reoxygenation, as a model relevant to the pathogenesis of ischemic injury, cultured rat astrocytes were exposed to hypoxia. On restoration of astrocytes to normoxia, there was a dramatic increase in protein synthesis within 3 h, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis of metabolically labeled astrocyte lysates showed multiple induced bands on fluorograms. Levels of cellular ATP declined during the first 3 h of reoxygenation and the concentration of AMP increased to almost-equal-to 3.6 nmol/mg of protein within 1 h of reoxygenation. Reoxygenated astrocytes generated oxygen free radicals early after replacement into ambient air, and addition of diphenyliodonium, an NADPH oxidase inhibitor, diminished the generation of free radicals as well as the induction of several bands on fluorogram. Although addition of cycloheximide on reoxygenation resulted in inhibition of both astrocyte protein synthesis and accumulation of cellular AMP, it caused cell death within 6 h, suggesting the importance of protein synthesis in adaptation of hypoxic astrocytes to reoxygenation. Potential physiologic significance of biosynthetic products of astrocytes in hypoxia/reoxygenation was suggestedby the recovery of glutamate uptake. These results indicate that the astrocyte response to hypoxia/reoxygenation includes generation of oxygen free radicals and de novo synthesis of products that influence cell viability and function in ischemia.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 15:14:20