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Titolo:
SEPARATE EFFECTS OF CAFFEINE AND DBCAMP ON MACAQUE SPERM MOTILITY ANDINTERACTION WITH THE ZONA-PELLUCIDA
Autore:
VANDEVOORT CA; TOLLNER TL; OVERSTREET JW;
Indirizzi:
UNIV CALIF DAVIS,CALIF REG PRIMATE RES CTR DAVIS CA 95616 UNIV CALIF DAVIS,SCH MED,DEPT OBSTET & GYNECOL,DIV REPROD BIOL & MED DAVIS CA 00000
Titolo Testata:
Molecular reproduction and development
fascicolo: 3, volume: 37, anno: 1994,
pagine: 299 - 304
SICI:
1040-452X(1994)37:3<299:SEOCAD>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-SPERMATOZOA; ACROSOME REACTION; FERTILIZING ABILITY; CAPACITATION; CALCIUM; PERCOLL; PENETRATION; CYNOMOLGUS; INDUCTION; AIH;
Keywords:
MACACA-FASICULARIS; SEMEN; CAPACITATION; ZONA BINDING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
C.A. Vandevoort et al., "SEPARATE EFFECTS OF CAFFEINE AND DBCAMP ON MACAQUE SPERM MOTILITY ANDINTERACTION WITH THE ZONA-PELLUCIDA", Molecular reproduction and development, 37(3), 1994, pp. 299-304

Abstract

Capacitation of macaque sperm with caffeine and dbcAMP is required for fertilization in vitro. This study determined the separate effects of caffeine and dbcAMP on sperm-zona pellucida binding and the acrosomereaction of zona bound sperm. Semen from 6 cynomolgus macaques was washed through 60% Percoll, resuspended, and washed with BWW media and incubated for 2.5 hr. Caffeine, dbcAMP (2 mM each), or both (1 mM each)were added to aliquots of the sperm suspensions. Immature macaque oocytes were placed into drops of sperm suspensions, coincubated with sperm for 30 sec, and either fixed immediately or removed to sperm-free media and incubated 1 hr before fixation. There were no significant differences between groups in the percentage of live, acrosome-reacted sperm in suspension. Treatment with caffeine and dbcAMP or with caffeinealone, significantly increased the number of sperm bound to each zonapellucida (96 +/- 16 and 81 +/- 17, respectively) compared to controland dbcAMP treatment (15 +/- 4 and 28 +/- 13). However, treatment with dbcAMP, alone and with caffeine, resulted in a higher percentage of acrosome-reacted sperm on the zona (15.2 +/- 2.1 and 9.0 +/- 0.6) thancontrol or caffeine treatment (3.0 +/- 1.4 and 2.4 +/- 0.5). Effects on sperm motility consistent with hyperactivation were detected only when both caffeine and dbcAMP were present. Although both caffeine and dbcAMP are presumed to increase or to produce the same effect as increased intracellular cAMP levels, these compounds have different effectson the ability of sperm to bind to the zona and to undergo the acrosome reaction. (C) 1994 Wiley-Liss, Inc.

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Documento generato il 01/12/20 alle ore 13:24:55