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Titolo:
DETECTION OF ROCHALIMAEA-HENSELAE DNA IN SPECIMENS FROM CAT-SCRATCH DISEASE PATIENTS BY PCR
Autore:
ANDERSON B; SIMS K; REGNERY R; ROBINSON L; SCHMIDT MJ; GORAL S; HAGER C; EDWARDS K;
Indirizzi:
CTR DIS CONTROL,NATL CTR INFECT DIS,DIV VIRAL & RICKETTSIAL DIS ATLANTA GA 30333 VANDERBILT UNIV,DEPT PEDIAT NASHVILLE TN 37232
Titolo Testata:
Journal of clinical microbiology
fascicolo: 4, volume: 32, anno: 1994,
pagine: 942 - 948
SICI:
0095-1137(1994)32:4<942:DORDIS>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
BACILLARY ANGIOMATOSIS; SP-NOV; PHYLOGENETIC-RELATIONSHIPS; BARTONELLA-BACILLIFORMIS; AGENT; PROTEOBACTERIA; THERAPY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
B. Anderson et al., "DETECTION OF ROCHALIMAEA-HENSELAE DNA IN SPECIMENS FROM CAT-SCRATCH DISEASE PATIENTS BY PCR", Journal of clinical microbiology, 32(4), 1994, pp. 942-948

Abstract

A PCR assay was developed by using degenerate primers that allow amplification of a 414-bp fragment of DNA from the rickettsia-like organisms Rochalimaea henselae and R. quintana. Internal oligonucleotides were used as hybridization probes, permitting rapid differentiation of these two Rochalimaea species. DNAs from 12 different isolates of R. henselae were amplified with the PCR primers, and the resulting 414-bp PCR product hybridized only with the R. henselae-specific probe. DNAs from four different isolates of R. quintana were amplified and produced a PCR product of the same size that hybridized only with the R. quintana-specific probe. DNAs from isolates of R. elizabethae, R. vinsonii, Bartonella bacilliformis, and Afipia felis failed to amplify the 414-bp fragment in the PCR assay. This two-step assay was applied to DNAs extracted from 16 fresh (unfixed) lymph node biopsy specimens and nine aspirates from patients with clinical cat scratch disease (CSI)) to assay for the presence of R. henselae or R. quintana DNA in these samples. Twenty-one (84%) of 25 lymph node samples from CSD patients were positive for R. henselae, while none were positive for R. quintana. The characteristic 414-bp fragment was not amplified from eight lymph nodetissue samples from non-CSD cases. These results provide evidence that R. henselae, and not R. quintana, plays the central role in the etiology of CSD.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 22:08:41