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Titolo:
EFFICIENCY OF POROUS HYDROPHILIC POLYMER-BASED PACKING MATERIALS IN CHROMATOGRAPHIC ANALYSIS OF DRUGS EXISTING WITH POLYPEPTIDE
Autore:
HOSOYA K; KISHII Y; KIMATA K; ARAKI T; TANAKA N;
Indirizzi:
KYOTO INST TECHNOL,DEPT POLYMER SCI & ENGN,SAKYO KU KYOTO 606 JAPAN
Titolo Testata:
Chromatographia
fascicolo: 3-4, volume: 38, anno: 1994,
pagine: 177 - 182
SICI:
0009-5893(1994)38:3-4<177:EOPHPP>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHASE SILICA SUPPORTS; LIQUID-CHROMATOGRAPHY;
Keywords:
COLUMN LIQUID CHROMATOGRAPHY; HYDROPHILIC POLYMER-BASED PACKING MATERIAL; DIRECT INJECTION OF SERUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
9
Recensione:
Indirizzi per estratti:
Citazione:
K. Hosoya et al., "EFFICIENCY OF POROUS HYDROPHILIC POLYMER-BASED PACKING MATERIALS IN CHROMATOGRAPHIC ANALYSIS OF DRUGS EXISTING WITH POLYPEPTIDE", Chromatographia, 38(3-4), 1994, pp. 177-182

Abstract

A size monodispersed restricted-access polymeric packing material hasbeen prepared through a simple and easy one-step method of co-polymerization of glycerol monomethacrylate and glycerol dimethacrylate with cyclohexanol as porogen. A typical seeded polymerization (two step swelling and polymerization method) in an aqueous medium gave a 90 % yield of porous beads which could be utilized as a packing material in high performance liquid chromatography (HPLC) without any size classification due to the excellent size monodispersity. A BET measurement and size exclusion chromatography in either tetrahydrofuran or water revealed that the prepared material had only small pores (around 100 Angstrom in diameter). It showed ample hydrophobicity for the separation of hydrophilic drug molecules in 10 % aqueous acetonitrile buffer, while polypeptides such as bovine serum albumin were excluded and eluted before the void volume of the column with quantitative recovery.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 04:31:00