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Titolo:
DOUBLE-LABELING TO OBTAIN S-PHASE SUBPOPULATIONS - APPLICATION TO DETERMINE CELL-KINETICS OF DIPLOID-CELLS IN AN ANEUPLOID TUMOR
Autore:
WHITE RA; POLLACK A; TERRY NHA; MEISTRICH ML; CAO S;
Indirizzi:
UNIV TEXAS,MD ANDERSON CANC CTR,DEPT BIOMATH,1515 HOLCOMBE BLVD HOUSTON TX 77030 UNIV TEXAS,MD ANDERSON CANC CTR,DEPT CLIN RADIOTHERAPY HOUSTON TX 77030 UNIV TEXAS,MD ANDERSON CANC CTR,DEPT EXPTL RADIOTHERAPY HOUSTON TX 77030
Titolo Testata:
Cell proliferation
fascicolo: 3, volume: 27, anno: 1994,
pagine: 123 - 137
SICI:
0960-7722(1994)27:3<123:DTOSS->2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
FLOW-CYTOMETRY; DNA; BROMODEOXYURIDINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
15
Recensione:
Indirizzi per estratti:
Citazione:
R.A. White et al., "DOUBLE-LABELING TO OBTAIN S-PHASE SUBPOPULATIONS - APPLICATION TO DETERMINE CELL-KINETICS OF DIPLOID-CELLS IN AN ANEUPLOID TUMOR", Cell proliferation, 27(3), 1994, pp. 123-137

Abstract

We studied the cell kinetics of the murine mammary carcinoma MCa-K using iododeoxyuridine (IdUrd) and chlorodeoxyuridine (CldUrd) given at different times as independently detectable labels of S phase cells. The presence of IdUrd and CldUrd, and the amount of DNA were measured by three-colour flow cytometry making it possible to define three subpopulations within S phase and to measure the progression through the cell cycle during the time following labelling. In DNA histograms of these subpopulations, the diploid and aneuploid cells (which had a DNA index of 1.7) are essentially completely separated. From appropriate combinations of cells labelled with IdUrd only, CldUrd only, or both, it was possible to construct separate DNA distributions for the labelled diploid and aneuploid cells at the times of administration of each label. The kinetics of the diploid and aneuploid cells could be calculated for individual tumours from these two time points without having to make corrections for the presence of the second population. The diploid and aneuploid populations had indistinguishable S and G(2)+M phase durations, T-S and T-G2+M, of about 9 and 2h; however, the potential doubling time values for the aneuploid and diploid populations were 30.2and 101.2h respectively.

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Documento generato il 02/07/20 alle ore 23:50:42