Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
THE ZINC-FINGER REGION OF THE ADENOVIRUS-E1A TRANSACTIVATING DOMAIN COMPLEXES WITH THE TATA BOX-BINDING PROTEIN
Autore:
GEISBERG JV; LEE WS; BERK AJ; RICCIARDI RP;
Indirizzi:
UNIV PENN,SCH DENT MED,DEPT MICROBIOL PHILADELPHIA PA 19104 UNIV PENN,GRAD GRP BIOL PHILADELPHIA PA 19104 UNIV CALIF LOS ANGELES,INST MOLEC BIOL,DEPT MICROBIOL & MOLEC GENET LOS ANGELES CA 90024
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 7, volume: 91, anno: 1994,
pagine: 2488 - 2492
SICI:
0027-8424(1994)91:7<2488:TZROTA>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
EUKARYOTIC TRANSCRIPTIONAL ACTIVATORS; E1A PROTEIN; TFIID COMPLEX; PROMOTERS; MUTANTS; DNA;
Keywords:
TRANSDOMINANT NEGATIVE MUTANTS; TRANSCRIPTION FACTORS; TFIID;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
J.V. Geisberg et al., "THE ZINC-FINGER REGION OF THE ADENOVIRUS-E1A TRANSACTIVATING DOMAIN COMPLEXES WITH THE TATA BOX-BINDING PROTEIN", Proceedings of the National Academy of Sciences of the United Statesof America, 91(7), 1994, pp. 2488-2492

Abstract

The 289R E1A protein of adenovirus transactivates a variety of viral and cellular promoters through protein-protein interactions. In earlier studies, mutational analyses of the E1A transactivating domain identified residues that are critical for transactivation and implied that the zinc finger region of the transactivating domain binds a transcription factor. Also, the E1A activation domain was found to bind to the TATA box binding protein (TBP) in vitro. Here, we tested the significance of the E1A-TBP interaction for E1A transactivation by analyzing the effects of conservative substitutions at each of the 49 residues of the E1A activation domain. Seven of the substitutions significantly diminished TBP binding in vitro. All of these were in the zinc finger region and were defective for transactivation in vivo. The perfect correlation between reduced TBP binding and transactivation argues stronglythat a direct interaction between the E1A activation domain and TBP is critical to the mechanism of E1A activation. This genetic analysis leads us to further suggest that another factor, which is limiting, is also necessary for E1A-mediated transactivation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 20:13:02