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Titolo:
EFFECT OF POTASSIUM-INDUCED DEPOLARIZATION AN SOMATOSTATIN GENE-EXPRESSION IN CULTURED FETAL-RAT CEREBROCORTICAL CELLS
Autore:
TOLON RM; FRANCO FS; DELOSFRAILES MT; LORENZO MJ; CACICEDO L;
Indirizzi:
HOSP RAMON Y CAJAL,SERV ENDOCRINOL,CARRETERA COLMENAR KM 9 E-28034 MADRID SPAIN HOSP RAMON Y CAJAL,SERV ENDOCRINOL E-28034 MADRID SPAIN INST CARLOS III,CTR NACL INVEST CLIN E-28034 MADRID SPAIN
Titolo Testata:
The Journal of neuroscience
fascicolo: 3, volume: 14, anno: 1994,
parte:, 1
pagine: 1053 - 1059
SICI:
0270-6474(1994)14:3<1053:EOPDAS>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROENKEPHALIN MESSENGER-RNA; CALCIUM-DEPENDENT RELEASE; CEREBRAL CORTICAL-CELLS; NERVE GROWTH-FACTOR; IMMUNOREACTIVE SOMATOSTATIN; MEMBRANE DEPOLARIZATION; CHROMAFFIN CELLS; PROTEIN-KINASE; BRAIN-CELLS; TRANSCRIPTION;
Keywords:
SOMATOSTATIN GENE REGULATION; CHRONIC DEPOLARIZATION; ACUTE DEPOLARIZATION; POTASSIUM; POTASSIUM-INDUCED SOMATOSTATIN GENE EXPRESSION; BRAIN SOMATOSTATIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
48
Recensione:
Indirizzi per estratti:
Citazione:
R.M. Tolon et al., "EFFECT OF POTASSIUM-INDUCED DEPOLARIZATION AN SOMATOSTATIN GENE-EXPRESSION IN CULTURED FETAL-RAT CEREBROCORTICAL CELLS", The Journal of neuroscience, 14(3), 1994, pp. 1053-1059

Abstract

The stimulatory effect of potassium depolarization upon somatostatin (SS) mRNA levels in primary cultures of fetal cerebrocortical cells was analyzed. Depolarizing stimuli, such as 56 mM K+ exposure for 30 min, elicited an increase in immunoreactive somatostatin (IR-SS) release to the media and decreased SS mRNA levels. These were increased when exposure to depolarization stimuli was prolonged up to 3 or more hr. Atthis time, potassium (30 and 56 mM) acted as a secretagogue, stimulating SS secretion, but was also effective in stimulating SS mRNA levels, suggesting that SS secretion can be coupled to SS mRNA accumulation. These changes were inhibited by the Ca2+ channel antagonist verapamil. In contrast, Na+ channel blockade by TTX did not modify the 24 hr potassium-induced increase in SS mRNA, although it partially abolished potassium-induced SS secretion. Examination of the rate of disappearance of SS mRNA levels after inhibition of mRNA transcription by actinomycin-D revealed that K+ stimulation of cerebrocortical cells stabilizedthe SS mRNA. These results suggest that the induction of SS mRNA expression by K+ is dose dependent, and involves the modulation of ion channels. The time-course study confirmed that the K+-induced SS mRNA accumulation is time dependent, chronic activation of the Ca2+ channels being necessary to stimulate SS gene expression. K+ stimulation may also increase the level of SS mRNA in cerebrocortical cells by reducing its rate of degradation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 05:19:04