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Titolo:
EFFECTS OF GONADOTROPIN-RELEASING-HORMONE (GNRH) ON THE CYTODIFFERENTIATION OF GONADOTROPES IN RAT ADENOHYPOPHYSEAL PRIMORDIA IN ORGAN-CULTURE
Autore:
KUDO A; PARK MK; KAWASHIMA S;
Indirizzi:
UNIV TOKYO,SCH SCI,INST ZOOL TOKYO TOKYO 113 JAPAN UNIV TOKYO,SCH SCI,INST ZOOL TOKYO TOKYO 113 JAPAN
Titolo Testata:
Cell and tissue research
fascicolo: 1, volume: 276, anno: 1994,
pagine: 35 - 43
SICI:
0302-766X(1994)276:1<35:EOG(OT>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTERIOR-PITUITARY; LH-CELLS; FETAL RATS; DIFFERENTIATION; INVITRO; CYTOGENESIS; MESENCHYME; RECEPTORS; INVIVO; SERUM;
Keywords:
ORGAN CULTURE; PITUITARY PRIMORDIUM; GONADOTROPES; LH; GNRH; RAT (WISTAR);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
A. Kudo et al., "EFFECTS OF GONADOTROPIN-RELEASING-HORMONE (GNRH) ON THE CYTODIFFERENTIATION OF GONADOTROPES IN RAT ADENOHYPOPHYSEAL PRIMORDIA IN ORGAN-CULTURE", Cell and tissue research, 276(1), 1994, pp. 35-43

Abstract

The effects of gonadotropin-releasing hormone (GnRH) on the development of gonadotropes were investigated by the use of organ culture and by means of immunocytochemistry and radioimmunoassay. Pituitary primordia from rat fetuses were cultured in a medium with or without 10(-9) MGnRH during the first 24 h of culture. The ratio of the number of immunoreactive LH cells to the total number of cells in the explants derived from 13.5-day fetuses was increased by the GnRH treatment after 6 or 8 days of culture, while the total number of cells was not altered. LH released into the medium and LH content of explants were not affected by the GnRH treatment. Subsequent treatment with 10(-9) M GnRH for4 h after 7 days of culture resulted in a marked release of LH, accompanying a significant decline in LH content, in both explants exposed or unexposed to the first GnRH treatment. However, the former explantscontained a lower amount of LH than the latter explants. The present results indicate that pituitary primordia at 13.5 days of gestation are capable to respond to GnRH, and that GnRH is effective in stimulating the responsiveness of gonadotropes to GnRH during early pituitary cytodifferentiation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 18:03:42