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Titolo:
ANTIISCHEMIC EFFECT OF DEFIBROTIDE TREATMENT IN RAT-KIDNEY
Autore:
CORSI M; PARISE M; GAJA G; FERRERO ME;
Indirizzi:
UNIV MILAN,IST PATOL GEN,VIA MANGIAGALLI 31 I-20133 MILAN ITALY UNIV MILAN,IST PATOL GEN I-20133 MILAN ITALY CNR,CTR CELLULAR PATHOL MILAN ITALY
Titolo Testata:
Drugs under experimental and clinical research
fascicolo: 6, volume: 19, anno: 1993,
pagine: 261 - 265
SICI:
0378-6501(1993)19:6<261:AEODTI>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
REPERFUSION INJURY; POSTISCHEMIC REPERFUSION; ISCHEMIA-REPERFUSION; METABOLIC FUNCTION; PROCUREMENT; DAMAGE; HEART; PROTECTION; LIVER; PGI2;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
17
Recensione:
Indirizzi per estratti:
Citazione:
M. Corsi et al., "ANTIISCHEMIC EFFECT OF DEFIBROTIDE TREATMENT IN RAT-KIDNEY", Drugs under experimental and clinical research, 19(6), 1993, pp. 261-265

Abstract

The authors previously demonstrated the protective activity of defibrotide (a profibrinolytic and antithrombotic drug) on endothelial cells. In the present work they examine the efficacy of defibrotide in protecting rat kidney from ischaemic/reperfusion injury by studying the modifications of intrarenal adenine nucleotide levels. Right renal ischaemia of 60 min and reperfusion of 30 min were induced in adult male Wistar rats. Defibrotide was administered as a bolus through a catheter inserted into the left femoral vein 5 min before the beginning of ischaemia at the dose of 32 mg/kg and continuously infused during ischaemia/reperiusion through the same vein al the final dose of 32 mg/kg in 5ml of saline at the rate of 3 ml/h. Rats treated with vehicle of the drug were used as controls. Al the end of postischaemic reperfusion, the ischaemic and left kidneys were rapidly removed and frozen in liquid nitrogen. Tissue extracts were prepared, and their ATP, ADP, AMP, cAMP, NAD(+), and NADH contents were determined by using luminescence methods. In controls, ATP intrarenal levels were significantly higher inthe left kidney than in the ischaemic organ of the same rat (3405 +/-320 vs. 378 +/- 36 nmol/g fresh tissue and mean +/- s.e.m. of 10 experiments). Defibrotide treatment significantly protected ischaemic kidneys from the drop in ATP intrarenal content (1465 +/- 147 vs. 3124 +/-303 nmol/g fresh tissue measured in the left kidney).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/08/20 alle ore 19:56:27