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Titolo:
INTRACELLULAR PH REGULATION IN RAT SCHWANN-CELLS
Autore:
NAKHOUL NL; ABDULNOURNAKHOUL S; KHURI RN; LIEBERMAN EM; HARGITTAI PT;
Indirizzi:
E CAROLINA UNIV,SCH MED,DEPT MED GREENVILLE NC 27858 E CAROLINA UNIV,SCH MED,DEPT PHYSIOL GREENVILLE NC 27858
Titolo Testata:
Glia
fascicolo: 3, volume: 10, anno: 1994,
pagine: 155 - 164
SICI:
0894-1491(1994)10:3<155:IPRIRS>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL-NERVOUS-SYSTEM; PROXIMAL TUBULE; EXTRACELLULAR PH; MUSCLE-FIBERS; GLIAL-CELLS; NEURONS; LEECH; H+; K+; COTRANSPORT;
Keywords:
HYDROGEN ION; PH(I); NA-H EXCHANGE; CL-HCO3; ABSORBENCY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
N.L. Nakhoul et al., "INTRACELLULAR PH REGULATION IN RAT SCHWANN-CELLS", Glia, 10(3), 1994, pp. 155-164

Abstract

We examined H+ and HCO3- transport mechanisms that are involved in the regulation of intracellular pH of Schwann cells. Primary cultures ofSchwann cells were prepared from the sciatic nerves of 1-3-day-old rats. pH(i) of single cells attached to cover slips was continuously monitored by measuring the absorbance spectra of the pH-sensitive dye dimethylcarboxyfluorescein incorporated intracellularly. The average pH(i) of neonatal Schwann cells bathed in HEPES mammalian solution was 7.17 +/- 0.02 (n = 32). In the nominal absence of HCO3-, pH(i) spontaneously recovered from an acute acid load induced by exposing the Schwann cells to 20 mM NH4+ (NH4+ prepulse). This pH(i) recovery from the acute acid load was totally inhibited in the absence of external Na+ or inthe presence of 1 mM amiloride. In both cases, the pH(i) recovery wasreadily restored upon readdition of external Na+ or removal of amiloride. In the steady-state, addition of amiloride caused a small and slow decrease in pH(i) which was readily reversed upon removal of amiloride. In the presence of HCO3-, removal of external Cl- caused pH(i) to rapidly and reversibly increase by 0.23 +/- 0.03 (n = 15) and the initial rate of alkalinization was 20.6 +/- 2.7 x 10(-4) pH/sec. In the absence of external Na+, removal of bath Cl- still caused pH(i) to increase by 0.15 +/- 0.02 and the initial rate of pH(i) increase was not significantly altered. In the nominal absence of HCO3- removal of bath Cl- caused pH(i) to increase very slightly (0.05 +/- 0.01) with an initial dpH(i)/dt of only 4.4 +/- 0.2 x 10(-4) pH/sec (n = 4). Addition of100 mu M DIDS did not inhibit the pH(i) increase caused by removal ofbath Cl-. These data indicate that 1) Rat Schwann cells regulate their pH(i) via an Na-H exchange mechanism which is moderately active at steady-state pH(i). 2) In the presence of HCO3-, there is a Na-independent Cl-HCO3 (base) exchanger which also contributes to regulation of intracellular pH in Schwann cells. (C) 1994 Wiley-Liss, Inc.

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Documento generato il 25/09/20 alle ore 10:52:11