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Titolo:
CYCLIN B1 AVAILABILITY IS A RATE-LIMITING COMPONENT OF THE RADIATION-INDUCED G(2) DELAY IN HELA-CELLS
Autore:
KAO GD; MCKENNA WG; MAITY A; BLANK K; MUSCHEL RJ;
Indirizzi:
UNIV PENN,SCH MED,DEPT PATHOL & LAB MED,JOHN MORGAN BLDG,ROOM 269,3620 HAMILTON WALK PHILADELPHIA PA 19104 UNIV PENN,SCH MED,DEPT PATHOL & LAB MED PHILADELPHIA PA 19104 UNIV PENN,SCH MED,DEPT RADIAT ONCOL PHILADELPHIA PA 19104
Titolo Testata:
Cancer research
fascicolo: 4, volume: 57, anno: 1997,
pagine: 753 - 758
SICI:
0008-5472(1997)57:4<753:CBAIAR>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
HAMSTER OVARY CELLS; IONIZING-RADIATION; MESSENGER-RNA; NITROGEN-MUSTARD; HUMAN WEE1; G(2)-PHASE ARREST; TYROSINE KINASE; P34CDC2 KINASE; DNA-DAMAGE; S-PHASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
48
Recensione:
Indirizzi per estratti:
Citazione:
G.D. Kao et al., "CYCLIN B1 AVAILABILITY IS A RATE-LIMITING COMPONENT OF THE RADIATION-INDUCED G(2) DELAY IN HELA-CELLS", Cancer research, 57(4), 1997, pp. 753-758

Abstract

Irradiation of tumor cells results in a G(2) delay, which has been postulated to allow DNA repair and cell survival, The G(2) delay after irradiation is marked in HeLa and other cells by delayed expression of cyclin B1. To test whether this depression of cyclin B1 contributes tothe G(2) delay, we induced cyclin B1 expression in irradiated HeLa cells using a dexamethasone-inducible promoter, Induction of cyclin B1 after radiation abrogated the G(2) delay by approximately doubling the rate at which the cells reentered mitosis, whereas dexamethasone itself had no effect, However, overexpression of cyclin B1 did not eliminate the G(2) delay in irradiated cells. In unirradiated cells, overexpression of cyclin B1 had no effect on cell cycle progression. Confirmation that reduction of cyctin B1 levels would prolong G(2) was provided using antisense oligonucleotides to cyclin B1. These results demonstrate that cyclin B1 levels control the length of the G(2) delay following irradiation in HeLa cells but do not exclude additional mechanisms controlling the mitotic delay after irradiation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 17:26:18