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Titolo:
AUTORADIOGRAPHIC MEASUREMENT OF TYROSINE-HYDROXYLASE IMMUNOREACTIVITYIN RAT-BRAIN USING ENHANCED CHEMILUMINESCENCE - A GENERAL-METHOD FOR COMBINED QUANTITATIVE AND CYTOCHEMICAL DETECTION
Autore:
NORMAN AB; KHOSLA UM; KLUG JM; THOMPSON HR;
Indirizzi:
UNIV CINCINNATI,COLL MED,DEPT PSYCHIAT,DIV NEUROSCI,231 BETHESDA AVE CINCINNATI OH 45267 UNIV CINCINNATI,COLL MED,DEPT ANAT & PHYSIOL,DIV NEUROSCI CINCINNATI OH 45267
Titolo Testata:
Brain research
fascicolo: 1-2, volume: 638, anno: 1994,
pagine: 352 - 356
SICI:
0006-8993(1994)638:1-2<352:AMOTI>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNOCYTOCHEMISTRY;
Keywords:
QUANTITATIVE IMMUNOCYTOCHEMISTRY; MONOCLONAL ANTIBODY; 6-HYDROXYDOPAMINE LESION; STRIATUM; SUBSTANTIA NIGRA; AUTORADIOGRAPHY;
Tipo documento:
Note
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
9
Recensione:
Indirizzi per estratti:
Citazione:
A.B. Norman et al., "AUTORADIOGRAPHIC MEASUREMENT OF TYROSINE-HYDROXYLASE IMMUNOREACTIVITYIN RAT-BRAIN USING ENHANCED CHEMILUMINESCENCE - A GENERAL-METHOD FOR COMBINED QUANTITATIVE AND CYTOCHEMICAL DETECTION", Brain research, 638(1-2), 1994, pp. 352-356

Abstract

Autoradiographic measurements of peroxidase labelled tyrosine hydroxylase immunoreactivity in brain sections from rats with unilateral 6-hydroxydopamine lesions were obtained using enhanced chemiluminescence. The autoradiograms required only 5-60 s exposure and displayed a good signal-to-noise ratio. The autoradiograms were quantitated using densitometry and by comparison with a standard curve. Following acquisitionof the autoradiograms the sections were stained with the chromogen diaminobenzidine (DAB). Distribution of the DAB closely followed the grain densities observed in the autoradiograms on both the lesioned and unlesioned side of the brain. This method represents a simple, rapid and inexpensive technique for performing both quantitative autoradiography and cytochemical studies in individual tissue sections of any peroxidase labelled immunoreactivity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 13:48:48