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Titolo:
IDENTIFICATION OF 3 N-LINKED GLYCANS IN THE V4-V5 REGION OF HIV-1 GP-120, DISPENSABLE FOR CD4-BINDING AND FUSION ACTIVITY OF GP-120
Autore:
HEMMING A; BOLMSTEDT A; JANSSON B; HANSEN JES; TRAVIS B; HU SL; OLOFSSON S;
Indirizzi:
UNIV GOTEBORG,DEPT CLIN VIROL,GULDHEDSGATAN 10B S-41346 GOTHENBURG SWEDEN UNIV GOTEBORG,DEPT CLIN VIROL S-41346 GOTHENBURG SWEDEN HVIDOVRE UNIV HOSP,DEPT INFECT DIS HVIDOVRE DENMARK BRISTOL MYERS SQUIBB PHARMACEUT RES INST SEATTLE WA 00000
Titolo Testata:
Archives of virology
fascicolo: 3-4, volume: 134, anno: 1994,
pagine: 335 - 344
SICI:
0304-8608(1994)134:3-4<335:IO3NGI>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; HAMSTER OVARY CELLS; GLYCOSYLATION SITES; AIDS VIRUS; ENVELOPE GLYCOPROTEINS; TRIMMING GLUCOSIDASE; DIRECTED MUTAGENESIS; SYNCYTIUM FORMATION; VIRAL INFECTIVITY; DISULFIDE BONDS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
A. Hemming et al., "IDENTIFICATION OF 3 N-LINKED GLYCANS IN THE V4-V5 REGION OF HIV-1 GP-120, DISPENSABLE FOR CD4-BINDING AND FUSION ACTIVITY OF GP-120", Archives of virology, 134(3-4), 1994, pp. 335-344

Abstract

Site-directed mutagenesis was used to study the biological significance of three N-linked glycans (linked to Asn406, Asn448, and Asn463), situated in the CD4-binding region of gp 120. Mutagenesis was carried out in a phage M13 system, and the mutated env genes were inserted intorecombinant vaccinia virus (r-vaccinia virus). To evaluate if the level of expression affected the biological phenotype of mutant gp 120, we expressed the envelope glycoproteins using either a weak (7.5 K) or a strong (11 K) promoter of vaccinia virus. The expression of mutated enu proteins was analyzed after infecting CD4-expressing HeLa cells with the r-vaccinia virus, by monitoring the ability of the infected cells to generate CD4-dependent syncytia. Env gene products lacking all three glycans as well as env gene products lacking different permutations of one or two glycans were analyzed. All mutated gp 120 species hadthe expected electrophoretical mobility as anticipated from elimination of one, two, and three N-linked glycans, respectively. Moreover, all mutant enu gene products demonstrated the same capacity to induce formation of syncytia, irrespective of using the weak or strong promoterfor expression. These data indicate that the three N-linked glycans studied are dispensable for HIV env gene products to function in CD4-binding and the subsequent fusion step.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 14:24:37