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Titolo:
ENVELOPE MEMBRANE-PROTEINS THAT INTERACT WITH CHLOROPLASTIC PRECURSORPROTEINS
Autore:
PERRY SE; KEEGSTRA K;
Indirizzi:
MICHIGAN STATE UNIV,MICHIGAN STATE UNIV DEPT ENERGY PLANT RES LAB E LANSING MI 48824 MICHIGAN STATE UNIV,MICHIGAN STATE UNIV DEPT ENERGY PLANT RES LAB E LANSING MI 48824 UNIV WISCONSIN,DEPT BOT MADISON WI 53706
Titolo Testata:
The Plant cell
fascicolo: 1, volume: 6, anno: 1994,
pagine: 93 - 105
SICI:
1040-4651(1994)6:1<93:EMTIWC>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
RIBULOSE-BISPHOSPHATE CARBOXYLASE; SMALL SUBUNIT; RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE; PHOSPHATE TRANSLOCATOR; INTACT CHLOROPLASTS; PEA-CHLOROPLASTS; IMPORT RECEPTOR; CONTACT SITES; TRANSPORT; BINDING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
S.E. Perry e K. Keegstra, "ENVELOPE MEMBRANE-PROTEINS THAT INTERACT WITH CHLOROPLASTIC PRECURSORPROTEINS", The Plant cell, 6(1), 1994, pp. 93-105

Abstract

The post-translational transport of cytoplasmically synthesized precursor proteins into chloroplasts requires proteins in the envelope membranes. To identify some of these proteins, label transfer cross-linking was performed using precursor to the small subunit of ribulose-1,5-bisphosphate carboxylase (prSSU) that was blocked at an early stage of the transport process. Two envelope proteins were identified: an 86-kDprotein and a 75-kD protein, both present in the outer membrane. Labeling of both proteins required prSSU and could not be accomplished with SSU lacking a transit peptide. Labeling of the 75-kD protein occurred only when low levels of ATP were present, whereas labeling of the 86-kD protein occurred in the absence of exogenous ATP. Although both labeled proteins were identified as proteins of the outer envelope membrane, the labeled form of the 75-kD protein could only be detected in fractions containing mixed envelope membranes. Based on these observations, we propose that prSSU first binds in an ATP-independent fashion to the 86-kD protein. The energy-requiring step is association with the75-kD protein and assembly of a translocation contact site between the inner and outer membrane of the chloroplastic envelope.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 21:42:30