Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
MUTANTS OF CHLAMYDOMONAS WITH ABERRANT RESPONSES TO SULFUR DEPRIVATION
Autore:
DAVIES JP; YILDIZ F; GROSSMAN AR;
Indirizzi:
CARNEGIE INST WASHINGTON,DEPT PLANT BIOL,290 PANAMA ST STANFORD CA 94305
Titolo Testata:
The Plant cell
fascicolo: 1, volume: 6, anno: 1994,
pagine: 53 - 63
SICI:
1040-4651(1994)6:1<53:MOCWAR>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEUROSPORA-CRASSA; MOLECULAR-CLONING; ARYLSULFATASE SYNTHESIS; NUCLEAR TRANSFORMATION; KLEBSIELLA-AEROGENES; SULFATE TRANSPORT; SACCHAROMYCES-CEREVISIAE; STRUCTURAL GENE; REGULATORY GENE; ARYL SULFATASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
J.P. Davies et al., "MUTANTS OF CHLAMYDOMONAS WITH ABERRANT RESPONSES TO SULFUR DEPRIVATION", The Plant cell, 6(1), 1994, pp. 53-63

Abstract

In the absence of sulfur, Chlamydomonas reinhardtii, a unicellular green alga, increases its rate of sulfate import and synthesizes severalperiplasmic proteins, including an arylsulfatase (Ars). These changesappear to help cells acclimate to a sulfur-deficient environment. Theelevated rate of sulfate import results from an increase in the capacity and affinity of the transport system for sulfate. The synthesis ofArs, a periplasmic enzyme that cleaves sulfate from aromatic compounds, enables cells to use these molecules as a source of sulfur when free sulfate is not available. To characterize the ways in which C. reinhardtii perceives changes in the sulfur status of the environment and regulates its responses to these changes, we mutagenized cells and isolated strains exhibiting aberrant accumulation of Am activity. These mutants were characterized for Ars activity, ars mRNA accumulation, periplasmic protein accumulation, and sulfate transport activity when grown in both sulfur-sufficient and sulfur-deficient conditions. All of the mutants exhibited pleiotropic effects with respect to several of these responses. Strains harboring double mutant combinations were constructed and characterized for Ars activity and ars mRNA accumulation. From the mutant phenotypes, we inferred that both positive and negative regulatory elements were involved in the acclimation process. Both theepistatic relationships among the mutations and the effects of the lesions on the responses of C. reinhardtii to sulfur limitation distinguished these mutants from similar mutants in Neurospora crassa.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/10/20 alle ore 12:02:24