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Titolo:
COMPARATIVE-EVALUATION OF THE ANTIPROLIFERATIVE EFFECT OF CYCLOSPORINE-A AND GAMMA-INTERFERON ON NORMAL AND HPV-TRANSFORMED KERATINOCYTES BY CELL COUNTING, MTT ASSAY AND TRITIATED-THYMIDINE INCORPORATION
Autore:
MARIONNET AV; LIZARD G; CHARDONNET Y; SCHMITT D;
Indirizzi:
HOP EDOUARD HERRIOT,INSERM U 346,PAVILLON R F-69437 LYON 03 FRANCE HOP EDOUARD HERRIOT,INSERM U 346 F-69437 LYON 03 FRANCE HOP BOCAGE,INSERM CJF 93 10 DIJON FRANCE
Titolo Testata:
Cell biology and toxicology
fascicolo: 2, volume: 13, anno: 1997,
pagine: 115 - 123
SICI:
0742-2091(1997)13:2<115:COTAEO>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLORIMETRIC ASSAY; GROWTH; CYTOTOXICITY; SUFFICIENT;
Keywords:
CELL GROWTH; CYCLOSPORINE A; INTERFERON-GAMMA, MTT ASSAY; NORMAL AND HPV-TRANSFORMED KERATINOCYTES; [H-3] TDR INCORPORATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
12
Recensione:
Indirizzi per estratti:
Citazione:
A.V. Marionnet et al., "COMPARATIVE-EVALUATION OF THE ANTIPROLIFERATIVE EFFECT OF CYCLOSPORINE-A AND GAMMA-INTERFERON ON NORMAL AND HPV-TRANSFORMED KERATINOCYTES BY CELL COUNTING, MTT ASSAY AND TRITIATED-THYMIDINE INCORPORATION", Cell biology and toxicology, 13(2), 1997, pp. 115-123

Abstract

We compared three techniques, the MTT tetrazolium assay, cell counting, and tritiated thymidine ([H-3]TdR) incorporation assay to measure the antiproliferative effect of cyclosporin A (CsA) and interferon-gamma (IFN-gamma) on normal human skin keratinocyte cultures (NHK) used atthe second passage and human papillomavirus type 16- and 18-transformed cell lines (EK16 and EK18) exposed continuously to the drugs for 3 days. The three techniques showed that under CsA (0.5 and 8 mu g/ml) and IFN-gamma (5 and 160 U/ml) treatments the cells remained viable andthat the growth of keratinocytes was inhibited. For IFN-gamma, the MTT colorimetric assay consistently underestimated its growth inhibitoryactivity as compared to cell counting or [H-3]TdR incorporation, whatever the cells used. For high doses of CsA, MTT and cell counting gavesimilar percentages of inibitory activity whatever the cells; MTT underestimated this activity as compared to [3H]TdR incorporation only inNHK and EK18 cells, whereas similar results were obtained with EK16 cells. In conclusion, this investigation shows that MTT sensitivity differed with the drug and also according to the keratinocyte cultures. The MTT test is clearly not appropriate for study of IFN-gamma treatment whatever the keratinocytes used. Such discrepancies indicate that the MTT test should be done with care on cultures to measure the effectsof drugs on cell growth; the growth inhibition should be carefully considered and it would be best if two different methods were used.

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Documento generato il 02/04/20 alle ore 02:06:58