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Titolo:
MUTATION DETECTION BY DENATURING GRADIENT ELECTROPHORESIS (DGGE)
Autore:
FODDE R; LOSEKOOT M;
Indirizzi:
MGC,DEPT HUMAN GENET,SYLVIUS LAB 2333 AL LEIDEN NETHERLANDS
Titolo Testata:
Human mutation
fascicolo: 2, volume: 3, anno: 1994,
pagine: 83 - 94
SICI:
1059-7794(1994)3:2<83:MDBDGE>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
SINGLE-BASE CHANGES; BETA-GLOBIN GENE; FACTOR-VIII GENE; FAMILIAL ADENOMATOUS POLYPOSIS; INSULIN-RECEPTOR GENE; HUMAN GENOMIC DNA; GEL-ELECTROPHORESIS; GC-CLAMP; MOLECULAR CHARACTERIZATION; POINT MUTATIONS;
Keywords:
DGGE; MUTATION; PCR; SSCP; HOT;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
94
Recensione:
Indirizzi per estratti:
Citazione:
R. Fodde e M. Losekoot, "MUTATION DETECTION BY DENATURING GRADIENT ELECTROPHORESIS (DGGE)", Human mutation, 3(2), 1994, pp. 83-94

Abstract

The molecular analysis of genetic diseases relies on several technical approaches which allow genetic and physical mapping, characterization of the gene structure, expression studies, and identification of disease-causing mutations. Denaturing gradient gel electrophoresis (DGGE)allows the rapid screening for single base changes in enzymatically amplified DNA. The technique is based on the migration of double stranded DNA molecules through polyacrylamide gels containing linearly increasing concentrations of a denaturing agent. In this review DGGE and the several modifications of the original protocol are presented. Moreover, its applications in human molecular genetics are summarized together with a preliminary comparison with other mutation detection technologies such as chemical cleavage, RNase protection, and single strand conformation polymorphism. (C) 1994 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 19:55:54