Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
DOUBLE-STAINING OF HORIZONTAL AND AMACRINE CELLS BY INTRACELLULAR INJECTION WITH LUCIFER YELLOW AND BIOCYTIN IN CARP RETINA
Autore:
TERANISHI T; NEGISHI K;
Indirizzi:
KANAZAWA UNIV,SCH MED,NEUROINFORMAT RES INST,DEPT NEUROPHYSIOL,1-13 TAKARA MACHI KANAZAWA ISHIKAWA 920 JAPAN NIPPON MED COLL,DEPT ANAT 2 TOKYO TOKYO 113 JAPAN
Titolo Testata:
Neuroscience
fascicolo: 1, volume: 59, anno: 1994,
pagine: 217 - 226
SICI:
0306-4522(1994)59:1<217:DOHAAC>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
INNER PLEXIFORM LAYER; FISH EUGERRES-PLUMIERI; GAP-JUNCTIONS; DENDRITIC MORPHOLOGY; TURTLE RETINA; BIPOLAR CELLS; DOPAMINE; CONDUCTANCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
T. Teranishi e K. Negishi, "DOUBLE-STAINING OF HORIZONTAL AND AMACRINE CELLS BY INTRACELLULAR INJECTION WITH LUCIFER YELLOW AND BIOCYTIN IN CARP RETINA", Neuroscience, 59(1), 1994, pp. 217-226

Abstract

Horizontal and amacrine cells in the isolated carp retina were impaled with micropipette electrode, identified by their characteristic light responses, and injected iontophoretically with markers for morphological study. Both Lucifer Yellow CH and biocytin were injected simultaneously. Lucifer Yellow was seen by its own fluorescence while biocytinwas visualized by binding with Texas Red-linked or horseradish peroxidase-conjugated avidin. For cone-connected horizontal cells, biocytin-coupled cells were found to be approximately five-times more numerous than Lucifer Yellow-coupled cells. Coupling for both tracers was consistently hampered by intravitreally applied dopamine. In untreated retinas, the injected Lucifer Yellow was restricted within one rod-connected horizontal cell, while biocytin revealed several coupled neighbors. Amacrine cells, labeled by the tracers, were morphologically grouped into eight types, based on our earlier classification.(29) Among them,amacrine cells, belonging to three types (Fnd, Pmb or Pma), were confirmed to be Lucifer Yellow-coupled, and the number of biocytin-coupledcells was more numreous (about 2.5 times) than that of Lucifer Yellow-coupled cells. Most amacrine cells (i.e. Pwd, Fnb and Fna) showed biocytin-coupling with no Lucifer Yellow-coupling. A few classified (i.e. Pwb and Fwa) and unclassified cells did not show any coupling. Since the tracer coupling takes place via gap junctions, the majority of amacrine cells, belonging to certain homologous types, appear to be functionally coupled with each other in the inner plexiform layer. However,dopamine did not influence the range of tracer coupling between amacrine cells in the carp retina under the present experimental conditions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 09:40:23