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Titolo:
OVEREXPRESSION OF HUMAN PROSTAGLANDIN G H SYNTHASE-1 AND SYNTHASE-2 BY RECOMBINANT VACCINIA VIRUS - INHIBITION BY NONSTEROIDAL ANTIINFLAMMATORY DRUGS AND BIOSYNTHESIS OF 15-HYDROXYEICOSATETRAENOIC ACID/
Autore:
ONEILL GP; MANCINI JA; KARGMAN S; YERGEY J; KWAN MY; FALGUEYRET JP; ABRAMOVITZ M; KENNEDY BP; OUELLET M; CROMLISH W; CULP S; EVANS JF; FORDHUTCHINSON AW; VICKERS PJ;
Indirizzi:
MERCK FROSST CTR THERAPEUT RES,DEPT PHARMACOL,POB 1005 POINTE CLAIRE H9R 4P8 PQ CANADA MERCK FROSST CTR THERAPEUT RES,DEPT MOLEC BIOL POINTE CLAIRE H9R 4P8 PQ CANADA MERCK FROSST CTR THERAPEUT RES,DEPT BIOCHEM POINTE CLAIRE H9R 4P8 PQ CANADA MERCK FROSST CTR THERAPEUT RES,DEPT MED CHEM POINTE CLAIRE H9R 4P8 PQCANADA
Titolo Testata:
Molecular pharmacology
fascicolo: 2, volume: 45, anno: 1994,
pagine: 245 - 254
SICI:
0026-895X(1994)45:2<245:OOHPGH>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRESSURE LIQUID-CHROMATOGRAPHY; ENDOPEROXIDE SYNTHASE; MESSENGER-RNA; H SYNTHASE; CDNA CLONING; CYCLOOXYGENASE; EXPRESSION; IDENTIFICATION; PROTEIN; CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
G.P. Oneill et al., "OVEREXPRESSION OF HUMAN PROSTAGLANDIN G H SYNTHASE-1 AND SYNTHASE-2 BY RECOMBINANT VACCINIA VIRUS - INHIBITION BY NONSTEROIDAL ANTIINFLAMMATORY DRUGS AND BIOSYNTHESIS OF 15-HYDROXYEICOSATETRAENOIC ACID/", Molecular pharmacology, 45(2), 1994, pp. 245-254

Abstract

Human prostaglandin G/H synthase (hPGHS)-1 and hPGHS-2, key enzymes in the formation of prostanoids from arachidonic acid, were expressed at high levels in COS-7 cells using a T7 RNA polymerase/vaccinia virus expression system. The open reading frame of hPGHS-2 cloned into vaccinia virus without its natural 5' and 3' untranslated regions directed only low levels of hPGHS-2 enzyme activity in COS-7 cells. High-level hPGHS-2 expression was achieved by appending the 3' untranslated region of hPGHS-1 to the hPGHS-2 open reading frame, with subsequent expression of the hybrid mRNA using vaccinia virus. Enzymatically active recombinant hPGHS-1 and hPGHS-2 were present as glycosylated proteins in the microsomal fraction prepared from infected cells, whereas recombinant hPGHS-1 and hPGHS-2 prepared from the microsomal fraction of cellstreated with tunicamycin, an inhibitor of N-linked glycosylation, were enzymatically inactive. The major prostanoid products formed by microsomes from COS-7 cells containing either recombinant hPGHS-1 or hPGHS-2 after incubation with arachidonic acid were prostaglandins D-2 and E(2), With lower levels of prostaglandin F-2 alpha, and 6-keto-prostaglandin F-1 alpha. A range of potencies were observed for various nonsteroidal anti-inflammatory drugs as inhibitors of prostaglandin E(2) synthesis by hPGHS-1 and hPGHS-2. Recombinant hPGHS-1 and hPGHS-2 both produced 15- and 11-hydroxyeicosatetraenoic acid (HETE) from arachidonic acid, with 15-HETE production by hPGHS-2 being stimulated 5-fold by preincubation with aspirin. Chiral phase high performance liquid chromatography analysis showed that aspirin-treated hPGHS-2 produced 15(R)-HETE, with no detectable 15(S)-HETE.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 05:24:22