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Titolo:
STIMULATION WITH A MONOCLONAL-ANTIBODY (MAB4E4) OF SCAVENGER RECEPTOR-MEDIATED UPTAKE OF CHEMICALLY-MODIFIED LOW-DENSITY LIPOPROTEINS BY THP-1-DERIVED MACROPHAGES ENHANCES FOAM CELL GENERATION
Autore:
HOLVOET P; PEREZ G; BERNAR H; BROUWERS E; VANLOO B; ROSSENEU M; COLLEN D;
Indirizzi:
UNIV LEUVEN,CTR MOLEC & VASC BIOL,CAMPUS GATHUISBERG O & N,HERESTR 49B-3000 LOUVAIN BELGIUM ACAD HOSP ST JAN,DEPT CLIN CHEM B-8000 BRUGGE BELGIUM
Titolo Testata:
The Journal of clinical investigation
fascicolo: 1, volume: 93, anno: 1994,
pagine: 89 - 98
SICI:
0021-9738(1994)93:1<89:SWAM(O>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHOLESTERYL ESTER ACCUMULATION; HUMAN ATHEROSCLEROTIC LESIONS; HUMAN MONOCYTE-MACROPHAGES; LIPID-PROTEIN ADDUCTS; APOLIPOPROTEIN-A-I; LINE THP-1; METABOLISM; LDL; DEGRADATION; FIBROBLASTS;
Keywords:
ATHEROSCLEROSIS; LOW DENSITY LIPOPROTEIN; MONOCLONAL ANTIBODIES; IMMUNE COMPLEXES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
63
Recensione:
Indirizzi per estratti:
Citazione:
P. Holvoet et al., "STIMULATION WITH A MONOCLONAL-ANTIBODY (MAB4E4) OF SCAVENGER RECEPTOR-MEDIATED UPTAKE OF CHEMICALLY-MODIFIED LOW-DENSITY LIPOPROTEINS BY THP-1-DERIVED MACROPHAGES ENHANCES FOAM CELL GENERATION", The Journal of clinical investigation, 93(1), 1994, pp. 89-98

Abstract

mAb4E4, a murine monoclonal antibody that is specific for acetylated LDL and malondialdehyde-treated LDL, binds specifically to modified LDI, present in human atherosclerotic lesions. It is directed against anepitope that is poorly exposed in delipidated and solubilized apolipoprotein B-100 from modified LDL. mAb4E4, as well as its P(ab')(2) and Fab fragments, enhanced the uptake of both acetylated LDL and malondialdehyde-treated LDL by THP-I-derived macrophages resulting in a sixfold increase of cytoplasmic cholesteryl ester levels. The increased uptake of modified LDL/mAb4E4 complexes did not occur via the Fc receptor and did not depend on aggregation of modified LDL particles. However, their uptake was inhibited by blocking the scavenger receptors with fucoidin or by down regulation of receptor expression with endotoxins orinterferon-gamma, indicating that their uptake is mediated via these receptors. Thus, generation of autoimmune antibodies against modified LDL and subsequent endocytosis of soluble modified LDL/antibody complexes via scavenger receptors may enhance foam cell generation. This mechanism may contribute to the progression of atherosclerotic lesions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/02/20 alle ore 21:26:05